A non-motile and rod shaped bacterium, designated strain B1(T), was isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C(18:1) omega9c; 42%) and hexadecanoic acid (C(16:0); 25.9%) and summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1) omega7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1(T) formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961(T) (98.6% sequence similarity), followed by A. baumannii DSM 30007(T) (97.4%), A. calcoaceticus DSM 30006(T) (97.0%) and 3 genomic species (96.8 approximately 7.6%). Phenotypic characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1(T) from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1(T) represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is B1(T) (= KCTC 22184(T)= JCM 15062(T)).
The growth responses of a grass, Poa pratensis, to elevated CO2 and nitrogen were investigated. Light-saturated photosynthetic rate per unit leaf area increased with exposure to elevated CO2, while dry weight did not respond to increased CO2. Patterns of biomass allocation within plants, including leaf area, leaf area ratio, specific leaf area, and root to shoot ratios, were not altered by elevated CO2, but changed considerably with N treatment. Shoot and wholeplant tissue N concentrations were significantly diluted by elevated CO2 (Tukey test, P < 0.05). Total N content did not differ significantly among CO2 treatments. The absence of a concomitant increase in N uptake under elevated CO2 may have caused a dilution in plant tissue [N], probably negating the positive effects of increased photosynthesis on biomass accumulation.
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