No abstract

The aim of this article is to study the indices of cellular, humoral immunity and nonspecific resistance, indices of free radical lipid oxidation and thrombocytoactive properties of periodontal tissues in animals with adjuvant periodontitis. In our study, adjuvant periodontitis was reproduced by the method of A.M. Kaminsky, immunizing rats with a homologous periodontal tissue in admixture with a Freund's adjuvant in a 1: 1 ratio. The course consisted of four single injections of 0.2 ml per animal intramuscularly at weekly intervals. Immunization did not lead to changes in the development and behavior of rats. One month after immunization, experimental animals experienced symptoms characteristic of generalized periodontitis in humans. They were manifested in the form of gums, resorption of alveolar bone, mobility and tooth loss. The induction of adjuvant periodontitis in experimental animals led to a significant increase in lipid peroxidation processes in periodontal tissues and a decrease in SOD and catalase activity. In animals with adjuvant periodontitis, which have been injected with periodontal polypeptides, there is a decrease in the reactions of GF compared with patients. In particular, there was a 40.9% decrease in the level of TBK-active products, although the level of MDA accumulation in the incubation process had not decreased significantly. It is noteworthy that in this group of animals increased activity of AO enzymes, which in patients was sharply reduced. Significant changes in the BPO lipid state were also observed in the blood of animals. The peroxidation reactions were significantly reduced, but not as significantly as in periodontal tissues. In particular, spontaneous erythrocyte hemolysis was lower by 14.2%, the level of TBK-active products by 39.4%, and MDA by 34.9%. An increase in the activity of SOD and catalase was observed, as well as a 40.4% decrease in the concentration of ceruloplasmin, which testifies to the elimination of the inflammatory response upon introduction of polypeptides. The treatment of animals with periodontylline was accompanied by an increase in the anti-aggregation properties of periodontal tissues. This is confirmed by the following indicators of the aggregate: the angle of aggregation is reduced by 31.8%, the optical density by 29%, the aggregation time is increased by 42.9% compared with the group of animals with adjuvant periodontitis. The introduction of parodontilin had a pronounced effect on the condition of blood clotting and fibrinolysis. We observed a significant increase in recalcification time, thrombin, prothrombin time. If the disease has a significant increase in the time of euglobulin fibrinolysis, then in the treatment of this indicator becomes less by 69.4%, the products of para-coagulation disappear from the bloodstream and the concentration of fibrin degradation products decreases. The treatment of animals was accompanied by an increase in immunity, which was manifested by an increase in T and B lymphocytes and Ig G. titer. Studies have shown that the treatment of diseased animals with a polypeptide drug leads to an improvement in the cellular, humoral immunity and nonspecific resistance of the organism, as well as the presence of a pronounced immunomodulatory effect of periodontin. The previously established relationship between blood clotting systems, sex, immunity, nonspecific resistance of the organism is confirmed in these experimental studies. If the induction of periodontitis is primarily associated with autoimmune processes, leading to impaired homeostasis, then the introduction of periodontin has a modulating effect on both immunity and indicators of lipid peroxidation and microcirculatory and coagulative hemostasis . In animals, regression of dental symptoms was noted, signs of periodontal inflammation, bleeding, swelling disappeared, and tooth mobility decreased. These data indicate the high therapeutic efficacy of thymic drugs in the treatment of generalized periodontitis. Research in this area remains relevant.

A dental cosmetic is actual for everyone. Whitening of teeth can be the method of lab treatmen. Whitening facilities which are used with this goal often contain peroxide, hydrogen, carbamide peroxide, and combinations of peroxides hydrogen with sodium perborate. The treatment of 3 patients with discoloration of non-vital teeth was done. In two cases the reason of color change was the use of paste containing resorcinol-formalin. Discoloring of root of the tooth was caused by necrotic damage of the pulp. Clinically satisfactory results were received after 1-4 visits in 1-2 days. Use of the bleaching method with further aesthetic restoration of tooth crowns allows avoiding significant weakening of the tooth crown in comparison with prosthetic methods of treatment. The main whitening agent was 30-40% hydrogen peroxide in combination with sodium perborate. The positive cosmetic results were achieved in short terms. Clear compliance with of manufacturer’s recommendations and the use of technology allow achieving success of treatment.

No abstract

A number of scientists put forward and confirmed the assumption of the presence in the body of biological regulators, polypeptides, carrying out the transfer of information necessary for the functioning, development and interaction of cell populations. The problem of studying the mechanism of action and clinical application of polypeptides is key in modern medicine. The aim of the research is to examine the clinical parameters and the state of free radical oxidation in blood and periodontal tissues of white rats in the treatment of spontaneous generalized periodontitis with the use of polypeptides. Materials and methods of the research. Experiments were performed on 280 six-month-old of rats Wistar line of both sexes weighing 120-130 g. During the study, the animals were kept under vivarium conditions in individual cells, food and water were not limited. All animals were divided into the following groups: Group I – intact animals (70), Group II – animals with spontaneous periodontitis (70), Group III – animals with spontaneous periodontitis, treated with thymalin polypeptide preparation in a dose of 0.1 mg / kg i / m daily, for 10 days (70), Group ІV – animals with spontaneous paradontitis, treated with parodontylin polypeptide preparation at a dose of 1 mg / kg i / m, daily for 10 days (70). Blood for research in rats was taken against the background of hexenalum anesthesia with a syringe from the heart into a plastic syringe. Subsequently, it was mixed with sodium citrate (3.8% solution) in a ratio of 9:1 and centrifuged at 1500 rpm for 10 min. Periodontal tissues and jaws of animals were taken and examined immediately after euthanasia. For the study, the collection of periodontal tissues (gums with periosteum) was performed in the area of the upper and lower molars, since periodontitis often affects these tissues. Indicators of free radical oxidation were determined in blood and periodontal tissues. Resorption of bone tissue of the alveolar process was evaluated. Results and discussion. In the treatment of experimental spontaneous periodontitis using thymalin on the 7th day of observations, regression of symptoms of the disease in animals was observed. Hyperemia and edema, bleeding gums remitted. Whereas under the influence of thymalin there is a regression of dental symptoms by 3.8 times, under the influence of parodontylin – by 7.2 times. The condition of periodontal tissues in animals improved more significantly on the 20th day of observation, the scale assessment of dental status indicates that in animals, treated with thymalin, it is 1.1, and by parodontylin – 0.3 points per animal. In studying the processes of lipid peroxidation in periodontal tissues, their significant reduction in rats with spontaneous periodontitis after administering parodontylin has been established. We also observed an increase in the activity of antioxidant enzymes. Hence, the activity of SOD was twice as high, catalase – by 1.95 times. Reactions of lipid peroxidation had the same dynamics in the blood of animals after administering periodontal polypeptides and thymalin, as in periodontal tissues. We observed a significant decrease in the level of conjugated dienes, the concentration of the latter reaches the value of indices of intact animals. Similar results were obtained with regard to the level of TBA-active products, accumulation of MDA, spontaneous hemolysis of erythrocytes. Attention is drawn to the fact that parodontylin reduced lipid peroxygenation of blood to a greater extent than thymalin. We also observed increased activity of SOD, and, on the other hand, the concentration of ceruloplasmin decreased by 25.4%. Thus, administering periodontal polypeptides during spontaneous periodontitis leads to a decrease in the responses of lipid peroxidation in periodontal tissues and blood. To a greater extent this effect is characteristic of parodontylin in comparison with thymalin.