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Effects of three preparations from porcine skin on the number of antibody-producing mouse splenocytes at the peak of primary immune response and on the restoration of the sensitivity of background rosette-forming splenocytes to inhibitory effect of azathioprine were studied in thymectomized mice. All three preparations were active in the first test, while in the second test only the preparation with molecular weight below 1.4 kD was active. The effects of preparations on B and T immunity have been demonstrated. Key Words: immunomodulators; cytokins; skin; B immunity; T immunityDevelopment of new immunomodulators is a perspective problem of modem immunology. Endogenous preparations, elements of natural metabolic processes, are of particular interest. The skin, a unique immune organ capable of developing local and systemic immune responses [4,6], is the source of immunomodulators. The overwhelming majority of cytokins were detected in the skin [4].We previously showed that porcine skin contains factors modifying the proliferation and differentiation of human keratinocytes in primary culture [5]. A method for isolation of these factors has been developed [1] and the physicochemical properties of these preparations were studied [2]. Here we investigated the effects of these preparations on some immunological parameters. MATERIALS AND METHODSPorcine skin was fragmented with scissors and homogenized in 0.14 M NaC1 (pH 7.0) at 4~ in a 1094 Homogenizer (Tecator). The homogenate was centrifuged at 2500g for 30 min at 4~ (J-6M/E centrifuge,