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Background: Many factors affecting satellitism test are not clear, and it is difficult to avoid misidentified even if the medium is properly selected. We investigated the factors that cause false positives of Haemophilus influenzae and false negatives of Haemophilus parainfluenzae in the satellitism test supplemented V factor by Staphylococcus aureus.Methods: H. influenzae (four reference strains and 47 clinical isolates), H. parainfluenzae (two reference strains and 67 clinical isolates), four different media, and two strains of S. aureus activated on two different media were involved in this study.Results: The type of medium used to activate S. aureus was the most common factor causing false positives of H. influenzae, followed by the strain of S. aureus, and again the type of medium used for the experiment. The production of false negatives of H. parainfluenzae was only related to the medium used in the experiment.Conclusions: Tryptic soy agar and S. aureus (ATCC 25923) activated with nutrient agar should be used in the satellitism test for Haemophilus spp. to improve the accuracy of the test.

In order to reduce the production cost of Microbial Flocculation，this article uses the cheap straw-inorganic nitrogen medium，directly domesticated and screened out a stable and efficient microbial flocculant producing strain B-15，which has better flocculation on kaolin clay suspension system. Preliminary identify as bacillu, the strain produce flocculant has flocculating rate of 81.08% for suspension.

Both the method of engineering and nonlinear finite element method have been used to the analysis and optimum design of the 30 in. marine riser with the screw thread. Two load steps have been used in the finite element analysis including the processes of both assembling and applying the load of piling, and the nonlinear contact between marine risers has been considered in the simulation. Numerical results show that the stress in the marine riser increase rapidly when the magnitude of interference of the screw thread change slightly. By the way to change interferential magnitude of the screw thread, the maximum stress of Von Mises decrease from 681MPa to 381MPa and contact stress decrease from 238MPa to 122MPa. The engineering practice over 100 piles in offshore platform has shown that the numerical results are good.

Understanding the mechanism of the forming of the welded pipe may help the engineer to design the shape of forming rollers and forming technology. It is hard to study the forming process of the welded pipe by both the method of experiment and numerical modeling because of too much nonlinear factors included in the forming process such as elastic-plastic large deformation and non linear contact. Although many research works have been conducted in this field by the method of experiment or numerical modeling, but few of the works deal with the whole forming process of the welded pipe. The main difficulties in the numerical modeling are of huge computational labor time, witch has been over the ability of the hardware and software of the computer. The whole process of roll forming of welded pipe has been simulated by nonlinear finite element method with ANSYS and LS-DYNA solver, the distribution of both stresses and strains have been got successfully. Mapping meshes and the rigid models for rollers have been used in the analysis in order to decrease the number of the elements. Numerical results and parameter study have shown that the forming rollers of both the level and vertical are the key factors to the forming process. It is believe that the method used in the paper can also be used to study the forming process of both cage roll forming and flexible forming.