Robotic gastrectomy could achieve extended lymph node dissection similar to open surgery. Our results showed a significant learning curve effect in the initial 25 cases of the robotic group.

No abstract

Lin28, a key factor for cellular reprogramming and generation of induced pluripotent stem cell (iPSC), makes a critical contribution to tumorigenicity by suppressing Let-7. However, it is unclear whether Lin28 is involved in regulating cancer stem-like cells (CSC), including in oral squamous carcinoma cells (OSCC). In this study, we demonstrate a correlation between high levels of Lin28B, Oct4, and Sox2, and a high percentage of CD44 OSCC cells was sufficient to enhanceOct4/Sox2 expression and CSC properties, whereas Let7 cooverexpression effectively reversed these phenomena. We identified ARID3B and HMGA2 as downstream effectors of Lin28B/ Let7 signaling in regulating endogenous Oct4 and Sox2 expression. Let7 targeted the 3 0 untranslated region of ARID3B and HMGA2 and suppressed their expression, whereas ARID3B and HMGA2 increased the transcription of Oct4 and Sox2, respectively, through promoter binding. Chromatin immunoprecipitation assays revealed a direct association between ARID3B and a specific ARID3B-binding sequence in the Oct4 promoter. Notably, by modulating Oct4/Sox2 expression, the Lin28B-Let7 pathway not only regulated stemness properties in OSCC but also determined the efficiency by which normal human oral keratinocytes could be reprogrammed to iPSC. Clinically, a Lin28B high -Let7 low expression pattern was highly correlated with high levels of ARID3B, HMGA2, OCT4, and SOX2 expression in OSCC specimens. Taken together, our results show how Lin28B/ Let7 regulates key cancer stem-like properties in oral squamous cancers. Cancer Res; 75(12); 2553-65. Ó2015 AACR.

BRAF mutation is an independent prognostic biomarker for colorectal liver metastasectomy.