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LncRNAs are increasingly verified to be aberrantly expressed in cancers. The abnormal expression of lncRNAs has turned out to be closely related to tumorigenesis or tumor progression. It has been reported that lncRNA OPI5-AS1 serves as a crucial regulator in tumors. However, the specific function of OIP5-AS1 on the progression of lung adenocarcinoma is still uncertain. In this paper, we mainly elucidated that OIP5-AS1 exerts oncogenic functions in human lung adenocarcinoma through targeting miR-448. We inspected that the expression of OIP5-AS1 was definitely high in lung adenocarcinoma tissues and cells, while miR-448 was sluggishly expressed in lung adenocarcinoma. OIP5-AS1 and miR-448 was negatively related to each other, the result was obtained from Pearson correlation analysis. We discovered a fact that OIP5-AS1 could directly sponge miR-448 through using dual luciferase reporter assay, RIP assay and RNA pull-down assay. Cell proliferation, migration and invasion were restrained after we disrupted the expression of OIP5-AS1 in lung adenocarcinoma. We also certified that OIP5-AS1 could sponge and regulate miR-448 to affect cell function in lung adenocarcinoma. MiR-448 could target Bcl-2 and affect the expression of Bcl-2. Then, we discovered that the expression of OIP5-AS1 and Bcl-2 was positively related. So we affirmed that lncRNA OIP5-AS1 modulated the expression of Bcl-2 by targeting miR-448 in lung adenocarcinoma cells.

Organic electrochemical transistors (OECTs) have emerged as versatile electrophysiological sensors due to their high transconductance, biocompatibility, and transparent channel material. High maximum transconductances are demonstrated facilitating the extracellular recording of signals from electrogenic cells. However, this requires large channel dimensions and thick polymer films. These large channel dimensions lead to low transistor densities. Here, interdigitated OECTs (iOECTs) are introduced, which feature high transconductances at small device areas. A superior device performance is achieved by systematically optimizing the electrode layout regarding channel length, number of electrode fingers and electrode width. Interestingly, the maximum transconductance (g max ) does not straightforwardly scale with the channel width-to-length ratio, which is different from planar OECTs. This deviation is caused by the dominating influence of the source-drain series resistance R sd for short channel devices. Of note, there is a critical channel length (15 µm) above which the channel resistance R ch becomes dominant and the device characteristics converge toward those of planar OECTs. Design rules for engineering the performance of iOECTs are proposed and tested by recording action potentials of cardiomyocyte-like HL-1 cells with high signal-to-noise ratios. These results demonstrate that interdigitated OECTs meet two requirements of bioelectronic applications, namely, high device performance and small channel dimensions.

To understand the physiology and pathology of electrogenic cells and the corresponding tissue in their full complexity, the quantitative investigation of the transmission of ions as well as the release of chemical signals is important. Organic (semi-) conducting materials and in particular organic electrochemical transistor are gaining in importance for the investigation of electrophysiological and recently biochemical signals due to their synthetic nature and thus chemical diversity and modifiability, their biocompatible and compliant properties, as well as their mixed electronic and ionic conductivity featuring ion-to-electron conversion. Here, the aim is to summarize recent progress on the development of bioelectronic devices utilizing polymer polyethylenedioxythiophene: poly(styrene sulfonate) (PEDOT:PSS) to interface electronics and biological matter including microelectrode arrays, neural cuff electrodes, organic electrochemical transistors, PEDOT:PSS-based biosensors, and organic electronic ion pumps. Finally, progress in the material development is summarized for the improvement of polymer conductivity, stretchability, higher transistor transconductance, or to extend their field of application such as cation sensing or metabolite recognition. This survey of recent trends in PEDOT:PSS electrophysiological sensors highlights the potential of this multifunctional material to revolve current technology and to enable long-lasting, multichannel polymer probes for simultaneous recordings of electrophysiological and biochemical signals from electrogenic cells.