Introduction The study assesses the effects of different insulation temperatures of an inflatable insulation system on right hemicolectomy. Materials and Methods A total of 132 patients receiving right hemicolectomy using the laparoscopic caudal approach were randomly divided into four groups. The inflatable insulation system was used immediately after they entered the operating room. The perioperative core body temperature, blood coagulation index, stress index, recovery time from anesthesia, and postoperative BIS recovery were recorded 5 minutes before induction of anesthesia (T0), immediately (T1), 30 minutes (T2), 60 minutes (T3), and 120 minutes after induction of general anesthesia (T4), and at the end of operation (T5). Results Group C had significantly lower IL1, IL-6, IL-23, IL-13, IL-17A, TNF-α, and CRP levels but a higher IL-12 level than those of other three groups (P < 0.05). The MAP and HR values of groups A and C were significantly lower than those of groups B and D at T2, T3 and T4, which were lowest in group C (P < 0.05). The blood glucose levels of groups A and C during and after operation were lower than those of groups B and D, being lowest in group C (P < 0.05). The anesthesia recovery time and extubation time of groups A and C were shorter than those of groups B and D, being shortest in group C (P < 0.05). The time from discontinuation to BIS>80 of group C was significantly shorter than those of other three groups (P < 0.05). Conclusions Comprehensive insulation (43°C automatically adjusted to 38°C after 1 hour) exerts satisfactory effects on right hemicolectomy.