Water is an important component of the ecosystem that sustains life on the planet since water provides vital components for human health. To gain a precise picture of water quality, studies of the components found in the water are critical. Three physical and six chemical criteria were examined to assess the quality assurance of drinking water of Majkhola. All tests were carried out according to the protocols outlined in the standard methodology. For this investigation, four water samples were collected in the morning from different areas of Majhkhola throughout three months, from December 2018 to February 2019. The purpose of this study was to evaluate the water quality parameters. pH (6.85 – 8.5), Conductivity (72.3 – 79.9 µm/s), Alkalinity (37.84 – 39.27 mg/L), Chloride (2.509 – 3.76 mg/L), Dissolves Oxygen (DO) (0.8 – 1.3 mg/L), Hardness (60 – 72 mg/L) and Total Dissolved Solids (100 – 150 mg/L) of water samples values lay within the permissible guideline values as prescribed by WHO and national standard but temperature (18 – 21°C) of water samples crossed the WHO permissible value and there is no prescribed limit of free CO2 (2 – 4) mg/L) in WHO. These metrics' values in the research area suggest that water samples are within normal or acceptable limits. As a result, water is safe to drink and meets local and international physical and chemical criteria to preserve excellent health. Based on the findings, it was concluded that the current study's findings are highly encouraging and reflect the health status of Majkhola's water area. Int. J. Appl. Sci. Biotechnol. Vol 10(1): 60-70.

Phosphate is primarily used as a builder in detergents, which helps soften hard water, thereby increasing the cleansing power of detergents. Extensive discharge of phosphate from detergents leads to eutrophication of surface water bodies. This work aims to assess the amount of phosphate present in detergent samples available in Tansen, Palpa, and compare its concentration between local and foreign detergents. Phosphate in detergent samples can be determined by the molybdenum blue phosphorus method using a spectrophotometer. The experimental process relies on forming a phosphomolybdate blue complex with ammonium molybdate and the subsequent reduction of the complex by hydrazine hydrate in an aqueous sulphuric acid medium. The system obeys Beer-Lambert’s law at 830 nm in the concentration range of 2-16 ppm. In this study, the phosphate concentration in local detergents (Dhoni, Light, V-Series, Nirma, Upahar, Patanjali, Rin Shakti and Dr Jhatka) varied from 15.54-33.03 ppm, and that of foreign detergents (Wheel, Super Check, Surf Excel, Tide and Ariel) ranged from 23.81- 32.65 ppm. The amount of phosphate was compared between local and foreign detergent samples using the student's t-test, which revealed no significant difference between them. The results obtained from the study disclosed that the detergents under investigation are within the permissible limits of phosphate. However, non- phosphate-based builders can be used to discourage the use of phosphate in detergents.