No abstract

The issue of urban-rural integrated development is a focus topic in China in academic and applied research. There is value in recognizing the formation of an urban-rural dual structure and in finding the causes of regional differences. Because Jiangsu is the central province in the coastal areas of eastern China, urban-rural integrated development in Jiangsu is an essential requirement for building a prosperous society and for ensuring the province's leadership role in accomplishing modernization. In this paper, the evaluation index system of Jiangsu province is assumed. The urban-rural integrated development level in the study area is measured by exploratory spatial data analysis, Markov chain and the principal component analysis method.The measured value is classified into higher, high, medium and low ranks. Next, the evolutionary characteristics of spatial and temporal patterns and the driving mechanism are explored. Spatial distribution of the urban-rural integrated development levels in Jiangsu province indicates that counties with higher levels are centred in southern Jiangsu and counties with high levels are located around the edge of higher level counties, signifying the spatial distribution of 'core-periphery'. The urban-rural integrated development levels appear to decrease from south to north. Counties with similar development levels tend toward spatial agglomeration. During the study period, the urban-rural integrated development levels of most counties improved to some degree. The directional distribution of polarization between areas with higher and low levels of development tends to diminish. Economic power, economic structure and information technology applications are the three major factors that exercise great influence over the spatial and temporal evolution of urban-rural integrated development levels in Jiangsu province. JEL classification: O18, R11

No abstract

TatD960 and TatD825 are DNases that contribute to biofilm formation and virulence in Trueperella pyogenes (T. pyogenes). Luteolin is a natural flavonoid commonly found in plants that exhibits antimicrobial capacity. Our study aims to investigate the effects of luteolin on TatD DNases as a natural inhibitor. In this research, the expression of tatD genes and TatD proteins in T. pyogenes treated with luteolin was detected, and then the effect of luteolin on the hydrolysis of DNA by TatD DNases was analyzed using agarose gel electrophoresis. Moreover, the interactions between luteolin and TatD DNases were tested using surface plasmon resonance (SPR) assays and molecular docking analysis. After 1/2 MIC luteolin treatment, the transcription of tatD genes and expression of TatD proteins appeared to be reduced in 80–90% of T. pyogenes (n = 20). The gel assay revealed that luteolin can inhibit the activity of TatD DNases. The SPR assay showed that the KD values of luteolin to TatD960 and TatD825 were 6.268 × 10−6 M and 5.654 × 10−6 M, respectively. We found through molecular docking that hydrogen bonding is predominant in the interaction of luteolin and TatD DNases. Our data indicate that luteolin inhibited the ability of TatD DNases by decreasing their binding to DNA. The current study provides an insight into the development of luteolin as a DNase inhibitor in preventing biofilm formation and virulence in T. pyogenes.

The EMD method was introduced to study the fluctuation of production and consumption of energy in China at multi-time scale from 1949-2008. Through the method of EMD, the fluctuations or trends in the different time level were unbundled to produce a series of different characteristics of the intrinsic mode functions and the system had the evolution curves on a different time scale, which could be as a special solution to the system. Results show that: the production and consumption of energy in China are separately decomposed into two IMFs and a trend by EMD method. The main periodic fluctuation of production of energy in China is about 13-year period and the secondary periodic fluctuation is about 4-year period. The main periodic fluctuation of consumption of energy in China is about 5-year period and the secondary periodic fluctuation is about 13year period. Then it analyzed the influence factors of the fluctuation of production and consumption of energy in China. Energy policy, economic development and industrial structure in common play important roles on the fluctuation of production and consumption of energy in China. Keywords-fluctuation; production and consumption of energy; empirical mode decomposition I.978-1-4244-9171-1/11/$26.00 ©2011 IEEE