The aim of this study was to evaluate the effect of surface roughness on the initial attachment of mouse osteoblast-like cells on ceria stabilized zirconia/alumina nanocomposite (NANOZR) and yttria-stabilized zirconia (3Y-TZP) in comparison to those on pure titanium (Ti) and alumina oxide (AO). Specimens with smooth and rough surfaces were prepared by grinding with diamond paper or by sandblasting, respectively. For four substrates examined, the number of attached cells on the rough surface specimens was significantly higher than that on the smooth surface specimens (p < 0.05). Integrin α5 and β1 expression had a greater increase in rough surface specimens than in smooth surface specimens. Actin cytoskeleton organization was, however, similar for both smooth and rough surface specimens. NANOZR and 3Y-TZP produced good cell attachment, similar to Ti and AO. The overall results demonstrated that NANOZR and 3Y-TZP with rough surface could provide good initial cell responses, adequate for future implant usage.

No abstract

No abstract

The purpose of this study was to evaluate the effects of acid-etched titanium on the biological responses of osteoblast-like MC3T3-E1 cells. Four types of treatments (polishing, sandblasting, concentrated H2SO4 etching, and concentrated H2SO4 etching with vacuum firing) were carried out on the surfaces of commercially pure titanium (cpTi) disks. MC3T3-E1 cells were then cultured on the treated cpTi surfaces. Through surface roughness measurement and SEM analysis, it was found that the acid-etched surfaces showed higher roughness values than the sandblasted ones. Scanning electron microscope analysis showed that the cells on the disks treated with acid-etching and acid-etching with vacuum firing spread as well as the sandblasted ones. There were no significant differences in cell proliferation and collagen production on cpTi among the four different surface treatments. Based on the results of this study, it was concluded that etching with concentrated sulfuric acid was a simple and effective way to roughen the surface of titanium without compromising its biocompatibility.