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A system using immobilized enzyme according to Oki et al., [Biol. Pharm. Bull. ,-(3) +*2.ῌ+*21 (,***)], which mimics the small intestinal membrane, was applied to the screening of several kinds of vegetables, fruits and herbs in terms of their inhibitory e#ects on a-glucosidase (AGH). AGH was partially purified from hog intestinal membrane by salting out, followed by immobilization on CNBr-activated Sepharose .B as a matrix. As substrate, p-nitrophenyl-aD -glucopyranoside (PNPG) was employed, producing p-nitrophenol (p-NP) by enzymic hydrolysis. Seven vegetables (Japanese radish, cabbage, onion, Chinese cabbage, tomato, cucumber, and carrot), / fruits (mandarin, apple, watermelon, melon, and grapefruit) and. herbs (Italian parsley, rucola, mache, and dill) were subjected to evaluations of the inhibitory e#ect on AGH. All of the test samples exerted an inhibitory e#ect on immobilized AGH (iAGH). Raw Chinese cabbage and boiled tomato among vegetables, mandarin among fruits and dill among herbs exerted the strongest inhibitory e#ect on iAGH.

BACKGROUND:The relationships between possible physiological properties of insoluble fibre and the viscosity of digesta are poorly understood. The aim of this study was to investigate the effect of insoluble fibres with different water-holding capacity, swelling, oil-holding capacity and cation exchange capacity on gastric, small intestinal and caecal contents in rats fed a semi-purified diet containing either no fibre (control), 50 g kg −1 tossa jute fibre or 50 g kg −1 shiitake fibre.

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