Hepatocellular carcinoma (HCC) is the most frequent primary liver cancer and has poor outcomes. However, the potential molecular biological process underpinning the occurrence and development of HCC is still largely unknown. The purpose of this study was to identify the core genes related to HCC and explore their potential molecular events using bioinformatics methods. HCC-related expression profiles GSE25097 and GSE84005 were selected from the Gene Expression Omnibus (GEO) database, and the differentially expressed genes (DEGs) between 306 HCC tissues and 281 corresponding noncancerous tissues were identified using GEO2R online tools. The protein-protein interaction network (PPIN) was constructed and visualized using the STRING database. Gene Ontology (GO) and KEGG pathway enrichment analyses of the DEGs were carried out using DAVID 6.8 and KOBAS 3.0. Additionally, module analysis and centrality parameter analysis were performed by Cytoscape. The expression differences of key genes in normal hepatocyte cells and HCC cells were verified by quantitative real-time fluorescence polymerase chain reaction (qRT-PCR). Additionally, survival analysis of key genes was performed by GEPIA. Our results showed that a total of 291 DEGs were identified including 99 upregulated genes and 192 downregulated genes. Our results showed that the PPIN of HCC was made up of 287 nodes and 2527 edges. GO analysis showed that these genes were mainly enriched in the molecular function of protein binding. Additionally, KEGG pathway analysis also revealed that DEGs were mainly involved in the metabolic, cell cycle, and chemical carcinogenesis pathways. Interestingly, a significant module with high centrality features including 10 key genes was found. Among these, CDK1, NDC80, HMMR, CDKN3, and PTTG1, which were only upregulated in HCC patients, have attracted much attention. Furthermore, qRT-PCR also confirmed the upregulation of these five key genes in the normal human hepatocyte cell line (HL-7702) and HCC cell lines (SMMC-7721, MHCC-97L, and MHCC-97H); patients with upregulated expression of these five key genes had significantly poorer survival and prognosis. CDK1, NDC80, HMMR, CDKN3, and PTTG1 can be used as molecular markers for HCC. This finding provides potential strategies for clinical diagnosis, accurate treatment, and prognosis analysis of liver cancer.

In wind tunnel tests, cantilever stings are often used as model-mount in order to reduce flow interference on experimental data. In this case, however, large-amplitude vibration and low-frequency vibration are easily produced on the system, which indicates the potential hazards of gaining inaccurate data and even damaging the structure. is paper details three algorithms, respectively, Classical PD Algorithm, Artificial Neural Network PID (NNPID), and Linear Quadratic Regulator (LQR) Optimal Control Algorithm, which can realize active vibration control of sting used in wind tunnel. e hardware platform of the first-order vibration damping system based on piezoelectric structure is set up and the real-time control software is designed to verify the feasibility and practicability of the algorithms. While the PD algorithm is the most common method in engineering, the results show that all the algorithms can achieve the purpose of over 80% reduction, and the last two algorithms perform even better. Besides, self-tuning is realized in NNPID, and with the help of the Observer/Kalman Filter Identification (OKID), LQR optimal control algorithm can make the control effort as small as possible. e paper proves the superiority of NNPID and LQR algorithms and can be an available reference for vibration control of wind tunnel system.

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Based on the Intelligent Vehicle Infrastructure Cooperative System (IVICS), the paper firstly analyzes the operating characteristics of the intersection group under real-time reversible lanes from the capacity; secondly, the signal phase sequence and signal timing parameters of the intersection are designed, and the green wave control is designed based on the phase difference model. The scheme provides traffic flowing in this section with a continuous green light signal. Finally, taking the intersection of Yongle East Road in Wuxi, Jiangsu Province as an example, it optimizes and controls the green wave of the intersection group and verifies the effectiveness of the phase difference model using the evaluation indicators of the number of stops, green wave speed, traffic capacity, and saturation flow. The results show that the optimization of the number of stops is 9.5% and the vehicle speed is optimized by 5.3%. The intersection capacity and saturation flow are greatly improved.