A new tobamo-like virus was isolated from a greenhouse-grown cucumber that showed severe mosaic distortion on leaves and fruit, in the southern part of Japan. The virus was tentatively designated Cucumber mottle virus (CuMoV) and further characterized. The size and antigenicity of the coat protein (CP) and the complete sequence of the genome were compared with those of the known cucurbit-infecting tobamoviruses: the W and SH strains of Cucumber green mottle mosaic virus (CGMMV), the C and Y strains of Kyuri green mottle mosaic virus (KGMMV), Cucumber fruit mottle mosaic virus (CFMMV), and Zucchini green mottle mosaic virus (ZGMMV). The CP of CuMoV migrated more slowly than those of CGMMV-W and -SH and KGMMV-C and -Y in sodium dodecyl sulfate polyacrylamide gel electrophoresis. In Western blot analysis, the CP of CuMoV cross-reacted weakly with antisera against CGMMV-W and did not react with antisera against KGMMV-Y. The overall nucleotide sequence of CuMoV had 62.5 to 63.5% identity with those of CGMMV-W, -SH, KGMMV-Y, CFMMV, and ZGMMV. The genome organization was characteristic of tobamoviruses, encoding a 131-kb protein, a 188-kb protein, a movement protein (MP), and CP in 5′ to 3′ order. In the phylogenetic analyses of the CP, CuMoV was placed in a separate lineage from CGMMV-W, -SH, KGMMV-C, -Y, CFMMV, and ZGMMV. The results indicate that CuMoV is a distinct tobamovirus species which represents a third sub-subgroup in the cucurbit-infecting tobamoviruses.

collected in Miyazaki with that found in Kagoshima and previously concluded that they were the same species, Colletotrichum capsici (Sydow) Butlar & Bisby, based on their morphology (Sato et al. 1999). We have since reidentified them as Colletotrichum dematium (Persoon: Fries) Grove based on their morphology and rDNA ITS-1 sequences.Symptoms. Many round, grayish green to dark brown spots 1-2 mm in diameter appear on lower leaves, and ellipsoid to fusiform and black spots are scattered on petioles and leaf vines at an early stage (Figs. 2, 3). The spots enlarge and coalesce to form light brown, irregular, large-lesions and then blight the entire leaflet (Fig. 2). The leaf blight progresses from the lower leaves to upper leaves, and whole plants develop the blight and finally fall to the ground. Numerous black acervuli with setae and milky to brownish orange masses of conidia formed on the lesions when the diseased plants were kept in moist conditions (Fig. 4). Although symptoms of Japanese radish anthracnose caused by C. higginsianum (Hagiwara and Kishi 1998) were similar to those mentioned here, damage by the present pathogen was more severe than that by C. higginsianum.Abstract Anthracnose of Japanese radish found in Kagoshima and Miyazaki prefectures was demonstrated to be caused by Colletotrichum dematium based on inoculation experiments and morphological and molecular identification of the pathogenic fungus. Although symptoms of Japanese radish anthracnose caused by Colletotrichum higginsianum were similar to those caused by C. dematium, damage by the latter pathogen was more severe than that by C. higginsianum.

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A method for controlling soilborne diseases using processed liquid from shochu distillery waste: control effect of Meloidogyne incognita infection of melon.

We show that the Temperley-Lieb algebra is constructed from the generators of the transversefield Ising-type bond algebra. It is also shown that, when we take the representation of the generators to the one-dimensional transverse-field Ising model, then the Temperley-Lieb algebra constructed on three consecutive bond generators becomes the XXZ model. Furthermore, we obtain several integrable spin systems from other representations for the bond generators.