Disinfectants have different efficacies depending on their use and the target microorganism. This study aimed to evaluate the efficacy and safety of our new nonalcoholic disinfectant, which consists mainly of metal ions. According to the 17th revised Japanese Pharmacopoeia and ASTM international E1052 method, the bactericidal and virucidal efficacy of this new disinfectant against 13 microorganisms was evaluated by the in vitro quantitative suspension test. Additionally, the disinfectant cytotoxicity against multiple cell lines was examined. Then, a safety test using a human open patch test was performed with 26 healthy volunteers. This disinfectant showed strong bactericidal and virucidal activities: all microorganisms except enterovirus were inactivated very quickly. The infectivity of 12 microbial strains was eliminated within 5 min of disinfectant exposure. Additionally, this disinfectant showed little acute cytotoxicity in vitro. All volunteers were negative in the human open patch test. Our new disinfectant has a broad spectrum of microbial targets, is safe for human skin, and demonstrates no cytotoxicity. This disinfectant could prevent common microbial infections.

Ti-6Al-4V alloy (Ti-Al-V) is used in dental implants and artificial hip joints to take advantage of its higher mechanical strength compared to cp Ti. However, the toxicity of vanadium and aluminum in the titanium alloy has been reported by several investigators since 1965. This study was carried out to compare the mechanical properties and the biocompatibility of Ti-Fe alloy (TiX®) to those of conventional metallic implant materials. The mechanical properties of TiX® were measured and compared with cp Ti, Ti-Al-V, SUS316, SUS304, Co-Cr-Mo, and Ag-Sn. The tensile strength of TiX® and Ti-Al-V were greater than those of Co-Cr-Mo, SUS316, and SUS304. The yield point of TiX® was slightly lower than that of Ti-Al-V, but higher than that of cp Ti. These results suggested the mechanical properties of TiX® were superior to those of Co-Cr-Mo, SUS316, and SUS304. To evaluate the biocompatibility, cell culture technique with L929 cells was used to investigate the cell multiplication, and SEM to investigate the morphological change of adhered cells onto TiX®. The cell growth rate of TiX® was slightly higher than those of Ti-Al-V and SUS316. Severe cytotoxicity was observed in Ag-Sn, and mild cytotoxicity in Ti-Al-V, SUS316, SUS304 using gyratory culture method. These results revealed that TiX® is a useful metallic material for wire, plate, porous, and mesh in dental and medical use, because of the favorable biocompatibility and easy fabrication.

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