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This article attempts to identify specific features of some commonly used Russian fruit and berry raw materials (raspberry, dewberry, gooseberry, dogrose, strawberry, cherry, kiwi, bananas) by estimating the content of total protein, its fractional and amino acid composition. It has been proven that among the studied fruit and berry raw materials, the most protein-rich fruit is banana (0.92%), while the lowest mass fraction of protein is observed in kiwifruit and cherries (0.49 and 0.51%, respectively). Further, in the rest of the fruit and berries, the mass fraction of protein is in the range between 0.53 and 0.81%. Studying the generic peculiarities showed that out of the studied raw materials, raspberries and gooseberries were rich in albumin (the mass fraction was 0.24 and 0.25%); the maximum number of glutelins was observed in gooseberry and banana (the mass fraction was 0.52 and 0.63%, respectively). In cherry and kiwi, the lowest albumin content was observed (0.09 and 0.07%, respectively). Despite the low mass fraction of globulins in the samples of raw materials, it was maximum in banana (0.09%), which, together with the high content of glutelins, ensured high enough contents of total protein (0.9%) in it. The experimental data obtained during analysis of protein in fruit and berry raw materials revealed that these fruits contain both nonessential (valine, leucine, isoleucine, lysine, methionine, threonine, tryptophan, phenylalanine) as well as essential (alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, proline, serine, tyrosine, cysteine) amino acids, and the content of these are varies as per the fruits types.

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Hypoxic ischaemic encephalopathy (HIE), an important complication of perinatal asphyxia, remains a major health burden. Neonates are classifi ed using the Sarnat score and neonates >35 weeks with moderate/ severe HIE are treated with therapeutic hypothermia (TH). TH improves survival/ decreases disability however 30-50% of mild HIE cases (not offered TH) develop cognitive impairment. Early biomarkers are vital to redefi ne HIE and better manage these infants. Cerebral monitoring is essential to establish cerebral activity, categorise encephalopathy stages, diagnose seizures, and monitor treatment. EEG and near infrared spectroscopy (NIRS) may be used concomitantly as monitoring tools (combined cerebral activity and haemodynamics) in order to study neurovascular coupling (NVC). NVC as a biomarker may increase sensitivity and specifi city for HIE outcome prediction. Limited research exists on NVC in neonates and in HIE where lower oxygen saturation may exist, physiological patterns may be altered. Easy integration of EEG and NIRS, continuous results over long periods, different clinical settings, favors studies. NVC analysis may identify neonates at risk earlier and reduce neurodevelopmental disabilities. AIM:-Study continuous EEG and NIRS data in HIE.-Measure NVC as biomarker for asphyxia severity -Identify/establish patterns of outcome prediction -Study effects of physiological variability and medication on NVC. METHODOLOGY:-Single center retrospective cohort study -UZ Leuven, Belgium: 10-year period 2010 -2020.-Establish database and inclusions.-Inclusion: 72 hours TH, EEG and NIRS, magnetic resonance imaging (MRI) < day 10 of life.-Demographic and clinical data (REDCap system) -Statistical analysis using GraphPad Prism.-MRIs scored blinded (Weeke HIE score). Score = 0 considered normal outcome, 1-2 a mild/moderate and >2 severe HIE. Comparisons between groups were performed.