Autor a quien debe ser dirigida la correspondencia Resumen Se presenta un estudio orientado a promover la sacarificación de la cascarilla de cebada a través de la aplicación de complejos enzimáticos comerciales. La cascarilla de cebada cervecera corresponde a un residuo lignocelulósico potencial para su introducción en procesos de biorrefinería dada su composición química y alto volumen de material residual. En este contexto, Viscozyme L, Celluclast 1.5L, Hemicellulase y Xylanase fueron evaluados en función de la dosificación con el objetivo de maximizar la respuesta en azúcares reductores. La hidrólisis enzimática presentó un efecto significativo obteniéndose los mejores resultados para el complejo Viscozyme L con una máxima liberación de glucosa de 16.9 g/L. Los resultados dan viabilidad a la implementación del uso del complejo Viscozyme L., permitiendo procesos más limpios y energéticamente eficientes. Palabras clave: residuo lignocelulósico; enzimas hidrolíticas; azúcares reductores AbstractA study aimed at promoting the saccharification of brewer's spent grain through the application of commercial enzymatic complexes is presented. Brewer's spent grain corresponds to a potential lignocellulosic residue for its introduction in biorefinery processes given its chemical composition and high volume of residual material. In this context, Viscozyme L, Celluclast 1.5L, Hemicellulase and Xylanase were evaluated according to the dosage in order to maximize the response in reducing sugars. Enzymatic hydrolysis presented a significant effect, obtaining the best results for the Viscozyme L complex with a maximum glucose release of 16.9 g/L.The results indicate that the implementation of the Viscozyme L complex is possible, allowing cleaner and energy efficient processes.
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