This work presents economical and ecological advances of microbiological utilization of inedible sources of plant biomass, procedure which is associated with bioethanol obtaining. We study influence of forced aeration and initial concentration of biomass of xylose-assimilating yeast P. tannophilus Y-1532/B2 on ethanol output from various xylose-containing substrates during periodical fermentation. The highest ethanol output is observed for OTR values equal to 5.0-8.0 mMole/l×h and yeast seeding density equal to 0.25 g a.d.s./g of substrate sugars. We show the possibility for intensification of ethanol obtaining technology from xylose-containing substrates. This was made using traditional biotechnological approaches of fermentation productions by optimization parameters of forced aeration of the fermentation medium and density of P. tannophilus biomass seeding. The obtained results might be used as initial parameters for calculation of laboratory regulations of complex microbiological utilization of secondary inedible sources of plant biomass of various origin and content. Industrial implementation of this technology will allow one to increase the economical coefficient of ethanol production from secondary inedible sources of plant biomass for 59.7-96.8% due to fermentation of D-xylose.