Harvested tomatoes were stored under atmospheres that were normoxic, anoxic, or modified (altered O2 and CO2 concentrations). Each atmosphere was created by storing the tomatoes at 25°C for up to 8 days in different kinds of pouches. During storage, metabolites of the tomatoes were measured using metabolomics. We obtained score plots of the metabolites on eighth day of storage by principal component analysis. There was a tendency for groups to be divided on the basis of score plot according to the composition of each gas. PC1 and PC2 seemed to correspond to the influence of O2 and CO2 concentrations, respectively, and the total contribution rate of the two axes was 72%, so that we concluded that the metabolites were affected mainly by O2 and CO2 concentrations. The results indicate that metabolomics may be an effective tool to reveal the relationship between metabolic state of harvested fruits and the atmosphere.

Eccentric exercise induces muscle damage and inflammation, resulting in a state of reduced physical activity with muscle dysfunction and a feeling of tiredness after exercise. Creatine is known to act as an energy buffer, but it has also been suggested to exert inhibitory effects on muscle damage and peripheral inflammation. The purpose of this study was to test the hypothesis that creatine supplementation alleviates fatigue after eccentric exercise and to explore the mechanism of this effect. C57BL/6J mice were fed an AIN-93G-formulated control diet or a creatine-containing diet for 6 days and were then subjected to downhill running, a model of eccentric exercise, to assess the effects on the total creatine concentrations in skeletal muscle and brain tissue, spontaneous activity, the urine concentration of titin N-fragment, and inflammatory gene expression. The results showed that creatine supplementation significantly increased the total creatine concentrations in skeletal muscle and brain tissue. Furthermore, spontaneous activity significantly decreased after downhill running and creatine supplementation maintained a significantly higher level of spontaneous activity. In addition, creatine supplementation significantly suppressed the downhill-running-induced increase in the mRNA expression of genes encoding ICAM-1, E-selectin, CD18, and BKB1R in the soleus muscle and IL-1β in the hypothalamus. On the other hand, creatine supplementation did not clearly influence the urine concentration of titin N-fragment. These results indicate that creatine supplementation may alleviate fatigue after eccentric exercise by partially suppressing inflammation in slow-twitch skeletal muscle and brain tissue.

The treatment of the animals was conducted according to the methods of Fukazawa et al. 7 . The animal room was maintained at 23 1 with 50 5 humidity and illumination from 09:00 to 21:00. Nine-week-old male Wistar rats Japan SLC, Inc. Shizuoka, Japan were individually housed in cages and were treated in accordance with the national guidelines for the care and use of laboratory animals Notification of the Prime Minister s Office of Japan . The NOTE Abstract: We previously reported that consuming a ketogenic diet containing medium-chain triacylglycerols (MCTs) might be a valuable dietary strategy for endurance athletes. However, the long-term safety of the diet has not been established, and there is a concern that a higher intake of MCTs increases the liver triacylglycerol content. In this study, we found that consuming an MCT-containing ketogenic diet for 24 weeks decreased, rather than increased, the liver triacylglycerol concentration and did not aggravate safetyrelated blood biomarkers in male Wistar rats. Our results may therefore suggest that the long-term intake of a ketogenic diet containing MCTs may have no deleterious effects on physiological functions.