The CO-sensing transcriptional activator CooA contains a six-coordinate protoheme as a CO sensor. Cys

No abstract

No abstract

These results suggest that the endothelin receptor of HASMCs is of the ETA type. We also studied the effect of ET-1 on the cytosolic [Ca 21]i in HASMCs loaded with fura-2/AM. In 1.3 mM Ca 2+, ET-1 produced a dose-depend ent, biphasic increase in signal with a maximal effect at 10 nM. At this concentration, ET-1 produced a transient increase in [Ca 21]i that reached a peak at 1 min, which was followed by a slow but sustained in crease in [Ca 2+]i. This second phase was attenuated in Ca 2+-deficient medium. Furthermore, ET-1 increased inositol 1,4,5-triphosphate in a time and dose-dependent manner. These results suggest that the endothelin receptors of HASMCs are of the ETA type, which couple with Ca 21 channels.

No abstract