1 To study the mechanisms involved in the action of Z-338, a newly synthesized gastroprokinetic agent, experiments were performed with the paratracheal ganglion cells acutely dissociated from 2-week-old Wistar rats. The e ects of Z-338 on both nicotinic and muscarinic responses of the ganglion cells were studied by nystatin perforated patch recording con®guration under the currentand voltage-clamp conditions. 2 Acetylcholine (ACh) or nicotine, and muscarine or oxotremorine-M (OX-M) induced membrane depolarization with rapid and slow time courses respectively, followed by repetitive generation of action potentials in the ganglion cell. Corresponding to the membrane depolarization induced by cholinergic agents, ACh induced biphasic inward currents with rapid and slow time courses under the voltage-clamp condition. Nicotine and muscarine or OX-M evoked inward currents with rapid and slow time courses, respectively. The rapid and slow inward currents were accompanied by increase and decrease in the membrane conductance, respectively. In addition, OX-M dosedependently suppressed the M-type K + current evoked in response to hyperpolarizing voltage-steps from V H of 725 mV to 750 mV, indicating that the activation of muscarinic acetylcholine receptors inhibits M-type K + current, thus inducing inward current in the ganglion cell. 3 Z-338 competitively suppressed the inward currents induced by OX-M through M 1 ACh receptor, and uncompetitively suppressed the currents induced by nicotine. 4 The inhibitory actions of Z-338 on the membrane depolarization and corresponding inward currents mediated by M 1 -muscarinic and neuronal nicotinic ACh receptors in the isolated ganglion cells were discussed in relation to the inhibitory actions on autoreceptors in the parasympathetic nerve terminals, which would explain the gastroprokinetic actions of Z-338.

1 The eects of the organotin, tri-n-butyltin (TBT), on N-methyl-D-aspartate (NMDA) induced membrane currents were investigated in order to evaluate possible neuronal actions of this toxic environmental pollutant. Experiments were conducted on neurons acutely dissociated from the rat dorsal motor nucleus of vagus (DMV) using the nystatin-perforated patch clamp recording technique. 2 In Mg 2+ -free physiological recording solutions, the application of NMDA to single DMV neurons held at a holding potential (V H ) of 740 mV evoked an inward current which rapidly reached a peak before declining to a steady-state inward current. This was followed, immediately after NMDA washout, by a transient outward current. TBT (100 nM) reversibly caused a slight reduction in the inward currents and greatly increased the amplitude of the outward currents. 3 The reversal potential of the NMDA-induced outward current in the presence of TBT was 786.7 mV, close to the theoretical K + equilibrium potential of 785.7 mV. 4 The NMDA-induced outward current was completely blocked when the K + in the internal solution was replaced with equimolar Cs + . Under these conditions, the NMDA induced current was more sustained and was unaected by TBT. 5 The NMDA-induced outward current was markedly inhibited by 5 mM tetraethylammonium chloride and 300 nM charybdotoxin, and it was abolished by removal of extracellular Ca 2+ , suggesting that the outward current was due to the activation of Ca 2+ -activated K + channels by Ca 2+ in¯ux through NMDA receptors. 6 In conclusion, in rat DMV neurons, TBT potentiates the Ca 2+ -activated K + current induced by NMDA application without having any direct eects on the NMDA-induced inward current. Given the signi®cant role of NMDA receptor mediated excitation in various physiological and pathological processes, the modulation of this response by TBT may have an important in¯uence on neuronal function.