Abstract. The paper tends to study the determination of formaldehyde in squid and bummalo by HPLC. The calibration curve in the 0.5-50.0 µg/mL range was obtained and correlation coefficient was 0.9993. The detection limit was 5 mg/kg. Endogenous formaldehyde in bummalo and squid as well as the change rule of formaldehyde under the frozen storage condition was assessed. Results showed that the formaldehyde content in bummalo was higher than the squid. Under the same storage conditions, formaldehyde content both in bummalo and squid were significantly increased in pace with frozen storage time. Therefore, inspection agencies should indicate the sampling time and testing time, when they detect the formaldehyde content in aquatic products.

Gold immunochromatography assay (GICA) technique has the following characteristics: rapid and simple, high sensitivity, good specificity, no auxiliary equipment, ease of interpreting results, and satisfactory stability. The technique has become one of the most rapid and sensitive immunological detection methods, which is widely used in medical, biological and other fields. The article focuses on the basic principles and technical characteristics of GICA, and briefly describes the applications and future perspectives in the rapid detection of aquatic products.

To investigate the hygienic quality of the lagoon seawater in Shanghai Jinshan City Beach (30°42 22 -30°43 06 N 121°20 40 -121°21 24 E) of North Hangzhou Bay, total coliform and fecal coliform was analyzed monthly from August 2006 to July 2007. The results showed that the densities of total coliform was no more than 1400MPN/L,the concentration is higher in July, August and September, the highest concentration of total coliform reached 1400MPN/L in September, after that, it began to decline. Monthly average fecal coliform was no more than 940MPN/L all the year round, likewise, it was relatively higher in July, August and September, but lower in winter and spring, with a lowest concentration of 20MPN/L,the highest concentration was 940MPN/L in September. From the regression analysis, it showed that coliform was positively correlated with fecal coli-group, their correlation coefficient was 0.7934. In addition, some environmental parameters including physical and chemical properties of seawater quality were also investigated. The environmental parameters were not positively correlated with both total coliform and fecal coliform. According to the Sea Water Quality Standard (GB3097-1997), the quality of seawater in Shanghai Jinshan City Beach was classified as the Second Grade and might be suitable for opening beach recreation park including swimming and other water direct contact activities.

No abstract

The pharmacokinetics of norfloxacin were investigated in the European eel after a single oral gavage of 10 mg norfloxacin per kg body weight. The concentrations of norfloxacin in the main tissues (kidney, muscle, hepatopancreas and blood) were simultaneously detected by HPLC. All of the concentration-time curves of norfloxacin in the plasma, muscle and liver were consistent with absorption of a two-compartment open kinetic model. Norfloxacin was widely distributed in different tissues in the European eel. Apparent volume of distribution (Vd) was 52.025 L/kg, 34.589 L/kg, 2.795 L/kg, and 0.969 L/kg, in plasma, muscle, liver and kidney, respectively. Norfloxacin in the eel was proved to eliminate slowly, and half-time (tβ1/2) in plasma, muscle, liver and kidney, was 201.222 h, 123.789 h, 120.634 h and 627473.495 h, respectively. Body clearance was 0.689 L / ( kg•h ), 1.793 L/( kg•h ), 0.097 L/( kg•h ) and 0.028 L /( kg•h ), in plasma, muscle, liver and kidney, respectively.