The proliferation of fake and inferior edible bird's nest (EBN) products has recently become an increasingly serious concern. To identify and classify EBN products, a competitive enzyme-linked immunoassay (ELISA) was developed to quantitate sialoglycoprotein in EBN used in food and cosmetic applications. The characteristic sialoglycoprotein in EBN was found, extracted, purified, and analyzed. Sialoglycoprotein, considered the main carrier of sialic acid in EBN, consisted of 106 and 128 kDa proteins. A monoclonal antibody that could recognize both proteins was prepared. The heat-treated process did not change the affinity of sialoglycoprotein with the antibody. An optimized ELISA method was established with a cross-reactivity of less than 0.1% and an IC(50) of 3.3 μg/mL. On the basis of different food and cosmetic samples, the limits of detection (LOD) were 10-18 μg/g. Recoveries of fortified samples at levels of 20 and 80 μg/g ranged from 81.5 to 96.5%, respectively. The coefficients of variation were less than 8.0%.

Traditional physical education methods are unable to meet this requirement due to the practical nature of sports skill teaching. As a result, as the times demanded, the flipped classroom based on neural network technology arose. It has the potential to not only promote the modernization of physical education but also to ensure that it has a positive educational impact. This is a mode of instruction. Furthermore, colleges and universities are increasingly focusing on college students’ overall quality development. A method for predicting college students’ sports performance using a particle swarm optimization neural network is proposed to accurately predict sports performance and provide a reliable analysis basis for the establishment of sports teaching goals. Neural networks are used in the model. The particle swarm optimization algorithm optimizes the variance and weights of the neural network to improve the accuracy of college students’ sports performance predicted by the neural network by updating the particle position and speed through the two extreme values of individual extreme values and global extreme values. Teachers always play the role of the facilitator and helper in the teaching process, which realizes the transformation of teachers’ and students’ self-positioning, allows students to better play the lead role, and stimulates students’ interest in learning.

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