This study provides genetic evidences at the chromosome, DNA content, DNA fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp 3 blunt snout bream, which belonged to a different subfamily of fish (Cyprininae subfamily and Cultrinae subfamily) in the catalog. We successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (RCC) ($) 3 blunt snout bream (BSB) (#) as well as their pentaploid hybrids. The triploid hybrids possessed 124 chromosomes with two sets from RCC and one set from BSB; the tetraploid hybrids had 148 chromosomes with two sets from RCC and two sets from BSB. The females of tetraploid hybrids produced unreduced tetraploid eggs that were fertilized with the haploid sperm of BSB to generate pentaploid hybrids with 172 chromosomes with three sets from BSB and two sets from RCC. The ploidy levels of triploid, tetraploid, and pentaploid hybrids were confirmed by counting chromosomal number, forming chromosomal karyotype, and measuring DNA content and erythrocyte nuclear volume. The similar and different DNA fragments were PCR amplified and sequenced in triploid, tetraploid hybrids, and their parents, indicating their molecular genetic relationship and genetic markers. In addition, this study also presents results about the phenotypes and feeding habits of polyploid hybrids and discusses the formation mechanism of the polyploid hybrids. It is the first report on the formation of the triploid, tetraploid, and pentaploid hybrids by crossing parents with a different chromosome number in vertebrates. The formation of the polyploid hybrids is potentially interesting in both evolution and fish genetic breeding.

A novel kind of array-assisted surface-enhanced Raman spectroscopy (SERS) microfluidic chip (ArraySERS chip) is demonstrated for gas sensing, which has the advantages of both ultrahigh sensitivity and multiplex sensing ability. On the one hand, the introduction of a microstructured triangular array can greatly increase the multiple collision probability between gas molecules and sensing interfaces in the channel. Compared with traditional gas sensors using sealed boxes, where gaseous molecules move only by diffusion, the ArraySERS chip exhibits significantly improved sensitivity. On the other hand, a composite nanoparticle is fabricated as a SERS probe for reading out the fingerprint spectral data, which consists of metal–organic framework (MOF) materials [Zeolitic Imidazolate framework-8 (ZIF-8)] and Au@Ag nanocubes, as well as cysteamine (CA) that serves as the gas-capturing agent. The experimental results show that such a structure of the SERS probe can further increase the sensing ability because of better adsorption of ZIF-8 for gas and the lower SERS background of CA itself. In addition, the simultaneous detection of multiplex gases was easily performed according to their own intrinsic SERS signals. Taking aldehyde gas as a model of a typical air pollutant, trace and multicomponent detection was realized using the ArraySERS chip. The limit of detection value was as low as 1 ppb, which is 2 magnitudes lower than that obtained by traditional methods. This strategy can be well extended for the detection of universal gases and help unleash the potential of existing gas sensors, especially for samples at low concentrations in air.

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In this article, sequence analysis of the coding region (5S) and adjacent nontranscribed spacer (NTS) were conducted in red crucian carp (RCC), blunt snout bream (BSB), and their polyploid offspring. Three monomeric 5S rDNA classes (designated class I: 203 bp; class II: 340 bp; and class III: 477 bp) of RCC were characterized by distinct NTS types (designated NTS-I, II, and III for the 83, 220, and 357 bp monomers, respectively). In BSB, only one monomeric 5S rDNA was observed (designated class IV: 188 bp), which was characterized by one NTS type (designated NTS-IV: 68 bp). In the polyploid offspring, the tetraploid (4nRB) hybrids partially inherited 5S rDNA classes from their female parent (RCC); however, they also possessed a unique 5S rDNA sequence (designated class I-L: 203 bp) with a novel NTS sequence (designated NTS-I-L: 83 bp). The characteristic paternal 5S rDNA sequences (class IV) were not observed. The 5S rDNA of triploid (3nRB) hybrids was completely inherited from the parental species, and generally preserved the parental 5S rDNA structural organization. These results first revealed the influence of polyploidy on the organization and evolution of the multigene family of 5S rDNA of fish, and are also useful in clarifying aspects of vertebrate genome evolution.

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