-In order to further purify two kinds of organic waste gases, methyl acetate and cyclohexane, which were difficult to be biodegraded in refinery wastewater treatment process, a new scheme for purifying mixed waste gas by bio-film construction with dominant bacteria was put forward. Because of the packing has a great influence on the performance of the bio-trickling filter (BTF) for purifying exhaust gas, the effect of two kinds of packing, namely ceramsite and volcanic rock, on cyclohexane purification was investigated. The results indicated that the filter efficiencies with two different packing were equivalent under the same conditions, but ceramsite was finally chosen as the next experimental packing because of its low weight. With activated sludge in secondary sedimentation tank from wastewater treatment plant as the original strain, the dominant degrading bacteria of methyl acetate and cyclohexane were obtained. After initial identification, a vertical BTF was startup with membrane of the dominant cyclohexane degradation bacteria Ochrobactrum intermedium. The tower can be startup quickly in 10 days, and the removal efficiency can reach over 95%. Moreover, the maximum eliminate capacity of cyclohexane was achieve 58.59 g·m -3 ·h -1 . When the reactor entered in stable phase, two kinds of methyl acetate predominant degrading bacteria, Bacillus velezensis and Ochrobactrum anthropi, were added and the methyl acetate gas was injected into the BTF. The performance of purify the mixture of cyclohexane and methyl acetate in the BTF was investigated in different operating conditions. The results showed that when the empty bed residence time was 176.6 s and 83.3 s, for cyclohexane concentration in the inlet air ranging from 50 mg·m -3 to 300 mg·m -3 , the removal efficiency was above 90%. Meanwhile, for methyl acetate concentration ranging from 300 mg·m -3 to 800 mg·m -3 , the removal efficiency was achieve 100%. The maximum eliminate capacity of the two kinds of pollutions was attain 72.99 g·m-3·h-1. In addition, the BTF was strongly resistant to impact load, and the pressure drop was remained at 5~100 pa over the entire operation time, no blockage appeared in the tower.