SummaryBiological membrane channels mediate information exchange between cells and facilitate molecular recognition1-4. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even more significant challenge is interfacing membrane channels with electronic devices for signal readout5-8. This challenge at the biotic-abiotic interface results in low efficiency of information transfer - one of the major barriers to the continued development of high-performance bioelectronic devices9. To this end, we integrate membrane spanning DNA nanopores with bioprotonic contacts to create programmable, modular, and efficient artificial ion-channel interfaces that resolve the ‘iono-electronic’ disparity between the biotic environment and electronics. Through simulations and experiments, we show that cholesterol modified DNA nanopores spontaneously and with remarkable affinity span the lipid bilayer formed over the planar bio-protonic electrode surface and mediate proton transport across the bilayer. Using the ability to easily modify DNA nanostructures, we illustrate that this bioelectronic device can be programmed for electronic recognition of biomolecular signals such as presence of Streptavidin, without disrupting the native environment of the biomolecule. We anticipate this robust biotic-abiotic interface will allow facile electronic measurement of inter-cellular ionic communication and also open the door for active control of cell behavior through externally controlled selective gating of the channels.

The ability to form robust, optoelectronically responsive, and mechanically tunable hydrogels using facile processing is desirable for sensing, biomedical, and light-harvesting applications. We demonstrate that such a hydrogel can be formed using aqueous complexation between one conjugated and one nonconjugated polyelectrolyte. We show that the rheological properties of the hydrogel can be tuned using the regioregularity of the conjugated polyelectrolyte (CPE) backbone, leading to significantly different mesoscale gel morphologies. We also find that the exciton dynamics in the long-time limit reflect differences in the underlying electronic connectivity of the hydrogels as a function CPE regioregularity. The influence of excess small ions on the hydrogel structure and the exciton dynamics similarly depends on the regioregularity in a significant way. Finally, electrical impedance measurements lead us to infer that these hydrogels can act as mixed ionic/electronic conductors. We believe that such gels possess an attractive combination of physical-chemical properties that can be leveraged in multiple applications.

Precise and highly regulated flow of biomolecules and ions through complex cellular networks is crucial for communication and information processing in living systems. In contrast, human‐made electronics rely on the flow of electrons and holes through well‐defined semiconductor networks for processing. Ion channels play vital roles in regulating the flow of ions and biomolecules across the cell membrane with a complexity unmatched in any semiconductor device. To enable biotic–abiotic communication and leverage this complexity, supported lipid bilayers (SLBs) create a planar cell membrane for integration with bioelectronics. This review discusses the integration of ion channels in bioelectronic devices for biotic–abiotic communication with enhanced functionality. This review begins with an introduction of natural and artificial ion channels across SLBs, continues with a description of bioelectronic devices integrating SLBs, and concludes with examples of functional ion channel bioelectronics.

Supported lipid bilayers (SLBs) are commonly used to investigate interactions between cell membranes and their environment. These model platforms can be formed on electrode surfaces and analyzed using electrochemical methods for bioapplications. Carbon nanotube porins (CNTPs) integrated with SLBs have emerged as promising artificial ion channel platforms. In this study, we present the integration and ion transport characterization of CNTPs in in vivo environments. We combine experimental and simulation data obtained from electrochemical analysis to analyze the membrane resistance of the equivalent circuits. Our results show that carrying CNTPs on a gold electrode results in high conductance for monovalent cations (K + and Na + ) and low conductance for divalent cations (Ca 2+ ).