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The diagnosis of suspected opportunistic infections in HIV patients is challenging due to the wide range of potential causes. This study used mNGS to analyse specimens of suspected opportunistic infections in HIV patients from a single centre to explore this method's applicability as a diagnostic tool compared to that of CMTs. Methods: We retrospectively investigated 46 suspected opportunistic infections in people living with HIV(PLWH) Hospitalized at Hangzhou Xixi hospital from January 2020 to August 2021. In total, we collected 49 samples (3 patients provided 2 samples) and sent them out for mNGS. Results: mNGS had a better detection rate for fungi and nontuberculous mycobacteria than that of CMTs. Specifically, the diagnostic detection rate of fungi (11 vs 19, P<0.05) and nontuberculous mycobacteria (1 vs 6, p<0.05) was significantly higher; there was no difference in detection rate for other pathogens (bacteria, Mycobacterium tuberculosis, or viruses). The sensitivity of mNGS was 90.91%, 50%, 0%, 100%, and 100% for detecting fungi, bacteria, Mycobacterium tuberculosis, nontuberculous mycobacteria, and viruses, respectively; the corresponding specificities were 74.29%, 97.73%, 86.36%, 86.67%, and 91.11%. Conclusion: mNGS technology provides an alternative and promising method of identifying suspected opportunistic infections in PLWH. Thus, the best diagnosis strategy may be using a combination of mNGS and CMTs.

Microscopic high-speed imaging is used to experimentally measure the velocity and size of droplets of gelled RP-1 based fuels with a solid particle additive. The gels are atomized using an air atomizing nozzle. The droplet diameter and velocity at a fixed position 20 cm from the nozzle on the centerline of the spray are measured at air mass flow rates of 1.5, 3 and 5 g/s. A parametric study is conducted to study the effect of gas mass flow rate, boron particle content, and species of the solid particle on the droplet characteristics. The results indicate that the droplet size decreases with the increasing of gas mass flow rate and boron particle content. Gel fuels with an aluminum particle are observed to produce smaller droplets at a low gas mass flow rate than that with a boron particle. The implication of these observations is that the atomization processes for gelled fuels with an additive of solid particles is controlled by the velocity difference between the gas and the droplets.

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