Puerarin (Pue) is the most abundant isoflavonoid in kudzu root. It has been widely used as a therapeutic agent for the treatment of cardiovascular diseases. However, poor-bioavailability of puerarin is the main obstacle to its widespread clinical applications. In this paper, HA-ADH-PS nanomicelles were prepared by chemical modification, noncovalent modification and etc, and characterized by means of FT-IR, ultraviolet (UV) and thermogravimetric analysis (TG). The encapsulation efficiency and drug loading of Pue-loaded HA-ADH-PS nanomicelles were 45.1% and 19.89% by UV, respectively. It could be observed from the transmission electron microscopy (TEM) images that HA-ADH-PS micelles appeared obvious spherical structure in the water. The particle size of HA-ADH-PS nanomicelles and Pue-loaded HA-ADH-PS nanomicelles were about 136.8 nm and 119.5 nm with a PDI of 0.237 and 0.272, respectively. The fluorescence probe method was used to characterize the critical micelle concentration, the critical micelle concentration (CMC) value of the nanomicells was 0.002 g/L and the results met the requirements and ensured the stability of micelles after dilution. DPPH assay suggested that Pue-loaded HA-ADH-PS nanomicelles had an obvious radical scavenging effect in vitro. MTT test showed that Pue-loaded HA-ADH-PS nanomicelles was non-toxic and had good biocompatibility. Thus, Pue-loaded HA-ADH-PS nanomicelles could be used as a potential drug carrier for puerarin.

Song (2020) Construction of poly(ethylene glycol)-poly(L-lactic acid)-stearic acid reverse aspirin-loaded micelles and optimization of preparation process,

Several kinds of composite materials with phosphotungstic acid (PTA) as the catalyst were prepared with activated carbon as support, and their structures were characterized. According to the Box–Behnken central combination principle, the mathematical model of the heterogeneous system is established. Based on the single-factor experiments, the reaction temperature, the reaction time, the amount of hydrogen peroxide and the loading capacity of PTA were selected as the influencing factors to study the catalyzed oxidation of hydrogen peroxide and degradation of high molecular weight chitosan. The results of IR showed that the catalyst had a Keggin structure. The results of the mercury intrusion test showed that the pore structure of the supported PTA catalyst did not change significantly, and with the increase of PTA loading, the porosity and pore volume decreased regularly, which indicated that PTA molecules had been absorbed and filled into the pore of activated carbon. The results of Response Surface Design (RSD) showed that the optimum reaction conditions of supported PTA catalysts for oxidative degradation of high molecular weight chitosan by hydrogen peroxide were as follows: reaction temperature was 70 ℃, reaction time was 3.0 h, the ratio of hydrogen peroxide to chitosan was 2.4 and the catalyst loading was 30%. Under these conditions, the yield and molecular weight of water-soluble chitosan were 62.8% and 1290 Da, respectively. The supported PTA catalyst maintained high catalytic activity after three reuses, which indicated that the supported PTA catalyst had excellent catalytic activity and stable performance compared with the PTA catalyst.