We propose a simple method of linearizing spectral interference fringes of spectral domain optical coherence tomography based on B-scan Doppler frequency shift (DFS), which can be obtained by offsetting the laser beam from the pivot of a scanning mirror. We show that DFS is proportional to wavenumber. A DFS based calibration curve can be then extracted from either a single mirror image or multiple tissue images. By examining the convergence of the nonlinear coefficients of the DFS curve fitted with a polynomial equation, tissue images themselves can be used to linearize the wavenumber without requiring mirror images or additional hardware. The method is verified with both ex vivo and in vivo tissue images. Using B-scan Doppler shift, we also demonstrate significantly improved imaging quality by combining complex conjugate ambiguity removal, speckle reduction, and spectral calibration with tissue images.

No abstract

No abstract

No abstract

No abstract