Background The Baiku Yao is a branch of the Yao nationality in China. The unique dying process of traditional clothing employed by these people has distinct national characteristics, a profound impact on the production and life of local people, and important research value. For this reason, it is important to investigate and document the dyeing plants and the traditional knowledge involved in the dyeing of Baku Yao traditional clothing. Methodology Information on dyeing plants was obtained using the free-list method and interviews with 61 Baiku Yao informants in Guangxi and Guizhou from January 2020 to August 2021. Based on the free-list method, we evaluated and screened out important plants by calculating the cognitive salience value of each plant. Results The results showed that the people of Baiku Yao have accumulated rich traditional knowledge of dyeing plants and long-term dyeing and other processes. We collected 23 species and recorded the related traditional knowledge, such as the Baiku Yao name, life form, habitat, part(s) used, application type, usage, and area used. The utilization of dyeing plants has a strong relationship with ethnic culture. The Baiku Yao uses unique anti-dyeing material (Ailanthus vilmoriniana) in the cotton dyeing process, they call it “the mother tree”. As well as, the results also showed that 15 plants (65.2%) have medicinal value and can be used to treat 18 aliments, and wild plants play a very important role in the life of the Baiku Yao. Conclusion These plants not only meet the color needs of the Baiku Yao people but also have health care effects, aligning with the trends toward green dyeing and the health contentiousness of modern people. This study provides a reference for the inheritance and development of Baiku Yao traditional clothing dyeing culture, will aid the identification of new natural, safe and healthy textile dyes, and is of positive significance in promoting regional economic development, national cultural inheritance, and biodiversity protection.

Zanthoxylum nitidum, known as Liang-Mian-Zhen in China, is a traditional Chinese medicinal plant used to treat traumatic injury, rheumatism, paralysis, toothache, stomach ache, and venomous snake bites. Two varieties of the species have been described and three morphological types have been reported within the original variety. However, taxonomic delimitation and molecular markers for distinguishing these varieties and types within this species remain unknown. Since different populations exhibit varying chemical compositions, easy identification of intraspecific taxa is crucial. We collected 420 individuals from 38 natural populations, 3 samples of standard medicinal material, and 17 folk-medicine samples to perform classification and identification within Zanthoxylum nitidum. Four distinct genetic clusters (A, B, C, and D) were highly supported by the nuclear barcode. Two distinct chloroplast clusters (A1 and A2) were further detected within A, and three others had one-to-one correspondence with the remaining nuclear clusters. Molecular identification showed that the 17 folk samples comprised A1, A2, B, and D, while the 3 standard samples belonged to A2. The internal transcribed spacer (ITS) region and rbcL gene are proposed as barcodes for rapid and accurate identification of the different Liang-Mian-Zhen lineages in China. This study highlights the importance of accurate taxonomic delimitation in combination with rapid and accurate molecular identification of medicinal plants.

Background: The Baiku Yao are a branch of the Yao nationality in China. The unique dyeing process of traditional clothing employed by these people has distinct national characteristics, a profound impact on the production and life of local people, and important research value. For this reason, it is important to investigate and document the dyeing plants and the traditional knowledge involved in the dyeing of Baiku Yao traditional clothing. This study provides a reference for the inheritance and development of Baiku Yao traditional clothing dyeing culture, will help to find new natural, safe and healthy textile dyes, and provides materials for green dyeing of modern clothing. At the same time, it is also expected that the research results will have positive significance in promoting regional economic development, national cultural inheritance and biodiversity protection. Methodology: Information on dyeing plants was obtained using the free-list method and interviews with 61 Baiku Yao informants in Guangxi and Guizhou from January 2020 to August 2021. Based on the free-list method, we evaluated and screened out important plants by calculating the cognitive salience value of each plant. Results: The results showed that the people of Baiku Yao have accumulated rich traditional knowledge of dyeing plants and the long-term dyeing and other processes. We collected 23 kinds of plants and recorded the related traditional knowledge, such as the Baiku Yao name, life form, habitat, part(s) used, application type, usage, and area used. The results also showed that 15 plants (65.2%) have medicinal value and can be used to treat 18 aliments, and wild plants play a very important role in the life of the Baiku Yao. Conclusion: These plants not only meet the color needs of the Baiku Yao people but also have health care effects, aligning with the trends toward green dyeing and the health contentiousness of modern people. This study will aid the identification of new natural, safe and healthy textile dyes and is of positive significance in promoting regional economic development, national cultural inheritance and biodiversity protection.

Zanthoxylum nitidum (Rutaceae Juss.) is a traditional Chinese medicine with four morphological types. We assembled their complete chloroplast genome sequences. The assembled genomes are 156,999–157,349 bp in length, including a large single copy (LSC) region of 84,064–84,455 bp, a small single copy (SSC) region of 17,582–17,651 bp and a pair of inverted repeats (IRs) of 27,631–27,659 bp. All genomes contained 133 genes, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. The GC content is 38.5%. The further phylogenetic analysis showed that Z. nitidum clade was a monophyletic group separated from other Zanthoxylum species.

Anoectochilus nandanensis and A. calcareus are medical plants with important economic value. In this study, we assembled their complete chloroplast (cp) genome sequences. The assembled cp genomes are 152,415 bp and 151,864 bp in length, including a large single-copy (LSC) region of 82,412 bp and 82,083 bp, a small single-copy (SSC) region of 17,367 bp and 17,142 bp and a pair of inverted repeats (IRs) of 26,318 bp and 26,320 bp, respectively. All genomes contained 133 genes, including 79 proteincoding genes, 30 tRNA genes, and 4 rRNA genes. The overall GC content of genomes in both species is 36.9%. Further phylogenetic analysis showed that three Anoectochilus comprised of a monophyletic clade and A. nandanensis was more close to A. emeiensis.