The cultivated land use eco-efficiency (CLUE) is an important indicator to evaluate ecological civilization construction in China. Research on the spatial-temporal pattern and evolution trend of the CLUE can help to assess the level of ecological civilization construction and reveal associated demonstration and driving effects on surrounding areas. Based on the perspective of the CLUE, this paper obtains cultivated land use data pertaining to National Pilot Zones for Ecological Conservation in China and neighboring provinces from 2008 to 2018. In this study, the SBM-undesirable, Moran’s I, and Markov chain models are adopted to quantitatively measure and analyze the CLUE and its temporal and spatial patterns and evolution trend. The research results indicate that the CLUE in the whole study area exhibited the characteristics of one growth, two stable, and two decline stages, with a positive spatial autocorrelation that increased year by year, and a spatial spillover effect was observed. Geographical spatial patterns and spatial spillover effects played a major role in the evolution of the CLUE, and there occurred a higher probability of improvement in the vicinity of cities with high CLUE values. In the future, practical construction experience should be disseminated at the provincial level, and policies and measures should be formulated according to local conditions. In addition, a linkage model between prefecture-level cities should be developed at the municipal level to fully manifest the positive spatial spillover effect. Moreover, we should thoroughly evaluate the risk associated with CLUE transition from high to low levels and establish a low-level early warning mechanism.