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The load and resistance factor design (LRFD) method is normally used to design B-regions of reinforced concrete (RC) flexural members. The design includes many checks corresponding to different limit states. The LRFD method requires many loop calculation steps in the design, demonstrating its relative inefficiency. It cannot be applied to compare limit states directly and quantitatively. Different design limit states are separated and isolated. How to improve the analytical calculation efficiency of the LRFD method and to realize direct and quantitative comparisons between limit states are very important problems in structural engineering. This paper presents an innovative unified flexural resistance design (UFRD) method and a unified flexural resistance evaluation (UFRE) frame to solve these problems to some extent. The main contents include the unified flexural resistance (UFR) principles, formulas for the unified flexural resistance design (UFRD) method, the operation procedure to facilitate its usage, the UFRE framework to compare limit states, and three examples. The results show that the UFRD method can provide the same design outcomes as the LRFD one. However, UFRD calculations are simpler, requiring at most 20% of the calculation steps of the LRFD method. The UFRE frame can make different limit states compare with each other directly and quantitatively, which cannot be realized by the LRFD method. It helps expose some potential and insufficient flexural resistance hazards for some limit states, such as the only 10% relative strength reservation of one example. Thus, the UFRD method and the UFRE frame supplement and develop the LRFD method to some degree. The simplicity and practicality of the approach and the frame make them appropriate for many applications.

Dietary starch with an increased content of resistant starch (RS) has the potential to reduce the prevalence of diabetes, obesity, and cardiovascular diseases. Here, an efficient glycogen branching enzyme, CcGBE, from Corallococcus sp. strain EGB was identified, and its relevant properties, including potential application in the preparation of modified starch, were evaluated. The purified CcGBE exhibited a maximal specific activity of approximately 20,000 U/mg using cassava starch as the optimal substrate. The content of α-1,6-glucosidic bonds in CcGBE-modified cassava starch increased from 2.9 to 13.2%. Meanwhile, both the average chain length (CL) of CcGBE-modified starch and the blue value of the color complex formed by starch and iodine initially increased and then decreased, indicating that a new CL transfer mode was reported. Perforated small starch granules were released after CcGBE treatment, and a time-dependent decrease in the retrogradation enthalpy (ΔH r) of cassava starch indicated that CcGBE inhibited the long-term retrogradation of starch. Moreover, the RS content and cold water solubility (CWS) of CcGBE-modified starch increased from 3.3 to 12.8% and from 23.1 to 93.8%, respectively. These findings indicate the application potential of CcGBE for the preparation of modified starch with increased RS and CWS.