The location of the infections is the basic data for precise prevention and control of dengue fever (DF). However, most studies default to residence address as the place of infection, ignoring the possibility that cases are infected at other places (e.g., workplace address). This study aimed to explore the spatiotemporal patterns of DF in Guangzhou from 2016 to 2018, differentiating workplace and residence. In terms of temporal and spatial dimensions, a case weight assignment method that differentiates workplace and residence location was proposed, taking into account the onset of cases around their workplace and residence. Logistic modeling was used to classify the epidemic phases. Spatial autocorrelation analysis was used to reveal the high and early incidence areas of DF in Guangzhou from 2016 to 2018. At high temporal resolution, the DF in Guangzhou has apparent phase characteristics and is consistent with logistic growth. The local epidemic is clustered in terms of the number of cases and the time of onset and outbreak. High and early epidemic areas are mainly distributed in the central urban areas of Baiyun, Yuexiu, Liwan and Haizhu districts. The high epidemic areas due to commuting cases can be further identified after considering the workplaces of cases. Improving the temporal resolution and differentiating the workplace and residence address of cases could help to improve the identification of early and high epidemic areas in analyzing the spatiotemporal patterns of dengue fever in Guangzhou, which could more reasonably reflect the spatiotemporal patterns of DF in the study area.