The success rate of in Vitro Fertilization (IVF) with ICSI depends on married couples factors such as maternal age, causes of infertility from the married couples, embryo status, reproductive history, lifestyle, ethnicity, and type of medical examination of infertility. The success rate of IVF was different among countries, rating from 21.4% to 51.7% meaning that the couples succeeded having a child, while in Indonesia the rate was unknown. Objective:To analyze infertility causing factors in married couples who underwent IVF and the success rate of IVF in one fertility center in Surabaya, Indonesia. Method: This observational study was conducting during January to December 2017. All couples who visited the fertility center,the female ≤ 39 years old, and the couples finished medical examination in the study place were asked to participate this study. Their characteristics (female age and duration of marriage), type infertility causing factors as well as the success rate.Results: Among 154 married couples, 64.3% of the female and 44.8% of the male had one or more abnormality in their reproductive status, while 26.6% of both (the couples) had the abnormalities, and 17.5% of both (the couples) had no abnormality (unexplained infertility). The success rate of IVF, meaning the female got pregnancy, was 37.7%.There was no significant association between male factors, female factors, more over the number of abnormalities were not associate to success rate. When correspondents divided in to 2 groups, having one or more abnormalities group vs. no abnormalities group (unexplained abnormality), we found that the success rate did not differ significantly between the two groups; suggesting additional examination were needed to predict the other factors for increasing the success rate in this study place.