Aim: This research aims to determine the effect of pre-birth counseling on women’s anxiety during the third trimester of pregnancy at Umbulharjo I Primary Health Center. Methods: This quantitative type of research used the pre-experiment method within Group Pretest-Posttest Design. Twenty people were used as a sample in this study, and they were the respondents who met the inclusion and exclusion criteria. The Accidental Sampling technique was used to take the sample. Results: This study found that 80% of the third-trimester-pregnant women experienced anxiety before the intervention, and there was a decrease in the anxiety of 25% after being given intervention. It was proven by the results of the p-value 0.011<0.05, meaning there is a positive effect of giving pre-birth counseling to lower the anxiety level of third-trimester-pregnant women. Thus, midwives are suggested to provide pre-birth counseling for pregnant women in Antenatal Care services. Keywords: third-trimester pregnancy, Anxiety