Chlorotic yellows disease of melon caused by Cucurbit chlorotic yellows virus, a new crinivirus. Jpn. J. Phyopathol. 75: 109-111.In 2004, an unidentified disease causing chlorotic yellows on the leaves of melon (Cucumis melo) plants was found in Kumamoto Prefecture. A few flexuous filamentous structures (ca. 900 nm in length) were observed with transmission electron microscopy. RT-PCR and subsequent nested PCR amplified a DNA fragment, which had 75% and 73% similarities with a corresponding sequence for Cucurbit yellow stunting disorder virus and Beet pseudo-yellows virus, respectively. Both Bemisia tabaci Q-biotype and B. tabaci Bbiotype (syn. B. argentifolii) acquired and transmitted the virus, and the infected melon plants developed the original symptoms of the disease. These results demonstrate that the virus is a new species in the genus Crinivirus (family Closteroviridae), and we propose the name Cucurbit chlorotic yellows virus.(
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