A simple, rapid, and robust RP-HPLC method have been developed and validated to measure palbociclib (PB) and letrozole (LT) at single wavelength (254 nm). A isocratic elution of samples performed on Intersil C (4.6 mm × 250 mm particle size 5 μm) column with mobile phase consisting 0.02 M sodium dihydrogen phosphate buffer (pH 5.5): acetonitrile: methanol (80:10:10 v/v/v) delivered at flow rate 1.0 mL min. A good linear response was achieved over the range of 5-50 μg mL. The LODs for PB and LT were found to be 0.098 and 0.0821 µg mL, while the LOQs for PB and LT were 0.381-0.315 µg mL, respectively. The method was quantitatively evaluated in terms of system suitability test, linearity, precision, accuracy (recovery) and robustness as per standard guidelines. The method is simple, convenient and suitable for the analysis of PB and LT in bulk drug.

Introduction: Diabetes mellitus, a metabolic disorder characterized by increased blood sugar level. Metformin hydrochloride is used to treat type I Diabetes mellitus. Metformin hydrochloride chemically 1, 1-dimethylbiguanide hydrochloride, is white crystalline powder, hygroscopic and freely soluble in water, Officially UV spectrophotometric method used for estimation of Metformin Hydrochloride from the bulk and tablets formulations. Objective: Develop and validate a simple, rapid, accurate, economic and precise UV/VIS method for Metformin Hydrochloride in bulk and tablets formulation. Methodology: Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed. Conclusion: Among different solvents water has showed better results, hence water was selected as a solvent for the proposed method. Metformin Hydrochloride showed maximum absorbance at 234 nm. The percentage recoveries for Metformin Hydrochloride were found in the range of 99-101 %. Method was quantitatively evaluated in terms of linearity, accuracy, precision, ruggedness, robustness and recovery. The method was simple, convenient and suitable for the determination of Metformin Hydrochloride from bulk and tablet dosage forms.

A simple, rapid, accurate, economic and precise UV/VIS method has been developed and validated. Choice of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed. methanol was selected as a solvent for the proposed method. Palbociclib and letrozole showed maximum absorbance at 220 and 240 nm respectively. Both drugs obey Beer Lambert’s law in the concentration range of 2-10 μg mL-1 for palbociclib and letrozole respectively. The LOD and LOQ were found to be 0.0782 μg mL-1and 0.2142μg mL-1for palbociclib, respectively. For Letrozole, the LOD and LOQ values were found to be 0.0585 μg mL-1and 1.6510μg mL-1 respectively. The method was quantitatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of palbociclib and letrozole in bulk drug.