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A high step-up dc-dc converter is proposed for photovoltaic power systems in this paper. The proposed converter consists of an input current doubler, a symmetrical switched-capacitor doubler and an active-clamp circuit. The input current doubler minimizes the input current ripple. The symmetrical switched-capacitor doubler is composed of two symmetrical quasi-resonant switched-capacitor circuits, which share the leakage inductance of the transformer as a resonant inductor. The rectifier diodes (switched-capacitor circuit) are turned off at the zero current switching (ZCS) condition, so that the reverse-recovery problem of the diodes is removed. In addition, the symmetrical structure results in an output voltage ripple reduction because the voltage ripples of the charge/pump capacitors cancel each other out. Meanwhile, the voltage stress of the rectifier diodes is clamped at half of the output voltage. In addition, the active-clamp circuit clamps the voltage surges of the switches and recycles the energy of the transformer leakage inductance. Furthermore, pulse-width modulation plus phase angle shift (PPAS) is employed to control the output voltage. The operation principle of the converter is analyzed and experimental results obtained from a 400W prototype are presented to validate the performance of the proposed converter.

The expression of potassium channels can be related to the occurrence and development of tumors. Their change would affect K+ outflow. Thus, in situ monitoring of extracellular K+ shows a great significance. Herein, the dual-functional K+ ion-selective electrode as the scanning electrochemical microscopy (SECM) tip (K+-ISE SECM tip) has been developed for in situ monitoring of the extracellular K+. Based on multi-wall carbon nanotubes as a transduction layer, the K+-ISE SECM tip realizes both the plotting of approach curves to position the tip for in situ detection and the recording of potential responses. It shows a near Nernstian response, good selectivity, and excellent stability. Based on these characteristics, it was used to in situ monitor K+ concentrations ([K+]o) of three breast cancer cell lines (MCF-7, MDA-MB-231, and SK-BR-3 cells) at 3 μm above the cell, and [K+]o of MDA-MB-231 cells show the highest value, followed by MCF-7 cells and SK-BR-3 cells. K+ outflow induced by electrical stimulation or pH changes of the culture environment (Δ­[K+]o) was further determined, and the possible mechanism of K+ outflow was investigated with 4-aminopyridin (4-AP). MCF-7 cells present the largest value of Δ­[K+]o, followed by MDA-MB-231 cells and SK-BR-3 cells at all the stimulation potentials, and pH 6.50 shows the greatest impact on K+ outflow of the three cell lines. The pretreatment of 4-AP changed K+ outflow, probably due to the regulation of voltage-gated channels. These findings provide insight into a deep understanding of the microenvironment influence on K+ outflow, thereby reflecting the possible mechanism of potassium channels.