Herein, a novel strategy was proposed for identifying
carbon monoxide
(CO), which plays a crucial part in living systems. For the first
time, we have managed to design, synthesize, and characterize successfully
this new Cu2+-assisted fluorescent probe (DPHP) in detecting CO. Compared with the commonly adopted Pd0-mediated Tsuji–Trost reaction recognition method, such a
new strategy did not engage costly palladium (II) salt and generated
no leaving group, indicating a satisfactory anti-interference ability.
The recognition mechanism was confirmed by IR, 1H NMR titration,
HR-MS, cyclic voltammetry, X-ray photoelectron spectroscopy, electron
paramagnetic resonance, and optical properties. Surprisingly, it was
found that the new method achieved high selectivity and rapid identification
of CO with a lower limit of detection (1.7 × 10–8 M). More intriguingly, it could recognize endogenous and exogenous
CO in HeLa cells. The cytotoxicity of this new method was so low that
it allowed the detection of CO in mice and zebrafish. Basically, our
results trigger a novel viewpoint of rationally designing and synthesizing
advanced materials for CO detection with unique features, impelling
new research in detection chemistry.
A new fluorescent
probe LXY based on the rhodamine
6G platforms has been designed, synthesized, and characterized, which
could recognize Fe3+ effectively in HEPES buffer (10 mM,
pH = 7.4)/CH3CN (2:3, v/v). And the distinct color change
and the rapid emergence of fluorescence emission at 550 nm achieved
“naked eye” detection of Fe3+. The interaction
mode between them was achieved by Job’s plot, MS, SEM, and
X-ray single-crystal diffraction. Importantly, the crystal structures
proved that Fe3+ could induce the rhodamine moiety transform
the closed-cycle form to the open-cycle form. But it is interesting
that Fe3+ did not appear in the crystal structures. Meanwhile,
the limit of detection (LOD) of LXY to Fe3+ was calculated to be 3.47 × 10–9. In addition,
the RGB experiment, test papers, and silica gel plates all indicated
that the probe LXY could be used to distinguish Fe3+ quantitatively and qualitatively on-site. Moreover, the
probe LXY has also been successfully applied to Fe3+ image in Caenorhabditis elegans, adult mice, and plant tissues. Thus, LXY was considered
to have some potential for application in bioimaging.
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