Reconstructing the colonization and demographic dynamics that gave rise to extant forests is essential to forecasts of forest responses to environmental changes. Classical approaches to map how population of trees changed through space and time largely rely on pollen distribution patterns, with only a limited number of studies exploiting DNA molecules preserved in wooden tree archaeological and subfossil remains. Here, we advance such analyses by applying high-throughput (HTS) DNA sequencing to wood archaeological and subfossil material for the first time, using a comprehensive sample of 167 European white oak waterlogged remains spanning a large temporal (from 550 to 9,800 years) and geographical range across Europe. The successful characterization of the endogenous DNA and exogenous microbial DNA of 140 (~83%) samples helped the identification of environmental conditions favouring long-term DNA preservation in wood remains, and started to unveil the first trends in the DNA decay process in wood material. Additionally, the maternally inherited chloroplast haplotypes of 21 samples from three periods of forest human-induced use (Neolithic, Bronze Age and Middle Ages) were found to be consistent with those of modern populations growing in the same geographic areas. Our work paves the way for further studies aiming at using ancient DNA preserved in wood to reconstruct the micro-evolutionary response of trees to climate change and human forest management.

Vegetation and lake-level data from the archaeological site of Tresserve, on the eastern shore of Lake Le Bourget (Savoie, France), are used to provide quantitative estimates of climatic variables over the period 4000-2300 cal BP in the northern French Pre-Alps, and to examine the possible impact of climatic changes on societies of the Bronze and early Iron Ages. The results obtained indicate that phases of higher lake level at 3500-3100 and 2750-2350 cal BP coincided with major climate reversals in the North Atlantic area. In west-central Europe, they were marked by cooler and wetter conditions. These two successive events may have affected ancient agricultural communities in west-central Europe by provoking harvest failures, more particularly due to increasing precipitation during the growing season. However, archaeological data in the region of Franche-Comté (Jura Mountains, eastern France) show a general expansion of population density from the middle Bronze Age to the early Iron Age. This suggests a relative emancipation of proto-historic societies from climatic conditions, probably in relation to the spread of new modes of social and economic organisation.

Pollen and sediment analyses were used to reconstruct vegetation and lake-level changes over the mid-Holocene period at Saint-Jorioz, Lake Annecy (northern French Pre-Alps). Episodes of forest clearings point to Neolithic cultural activities in the northern French Pre-Alps from c. 6500 cal. BP in agreement with other pollen and macrofossils records from eastern Switzerland and the Jura mountains. The lake-level record shows rises atc. 8300–8200, 6400, 5900 and after 5730 cal. BP. The rise at c. 8300–8200 cal. BP shows a tripartition. Lower water levels developed before 8300–8200 and 6665 cal. BP, at c. 6050 and 5730 cal. BP. Rises at c. 8300–8200, 6400, 5900 and after 5730 cal. BP can be related to the 8200-yr event and North Atlantic ice-rafted debris (IRD) events occurring at c. 6200, 5800 and 5500 cal. BP, respectively. Using a model based on pollen and lake-level data, the palaeoenvironmental changes reconstructed at Saint-Jorioz were translated into quantitative climate parameters. The results suggest that rises in lake level coincided with increasing annual precipitation, runoff and available moisture, and decreasing mean summer temperature and shortening of the growing season probably as a result of alternate southward/northward displacements of the Atlantic Westerly Jet. At Saint-Jorioz, the period around the 8200-yr event corresponded to a c. 2.5°C summer temperature cooling and a c. 130 mm P-E (precipitation-evaporation) increase in agreement with other European and marine palaeoclimate records; a c. 4°C summer-temperature cooling and a rise in annual precipitation by c. 175 mm are reconstructed at c. 5900 cal. BP and after 5730 cal. BP, i.e., close to the time of the North Atlantic IRD events dated at 5800 and 5500 cal. BP, and are to be tested by further investigations. A general trend toward temperature cooling and precipitation increase appears over the period documented by the sediment sequence of Saint-Jorioz possibly related to an orbitally induced reduction of summer insolation.

International audienceIn order to better understand the evolution of past climate-human-environment interactions in the North-western Alps during the Holocene, we have analysed the lipid content of two cores taken from the sediments of Lake le Bourget (French Alps). By using a specific molecular biomarker of Panicum miliaceum (broomcorn millet) previously defined and a new molecular marker of soil erosion, we demonstrate that the onset of millet cultivation coincides with the onset of major soils erosion in the catchment during the Middle Bronze Age. Although archaeological and archaeobotanical investigations indicate a discrete human occupation of the lakeshores at this period, they also point to a regional change in agricultural practices that deeply affected soils. The evolution of millet cultivation appears in strong connection with climatic variations, estimated in the same cores from the variations in titanium, a proxy of hydrological changes in the region. Social and cultural triggers cannot be discarded at this stage. Such an approach applied to more sedimentary archives shows high potential to unravel the temporal and spatial dynamics of human land-use

On the basis of sedimentological analysis of two cores taken at Chatillon, Lake Le Bourget (northern French Pre‐Alps), and well dated by radiocarbon dates in addition to tree ring dates obtained from an archaeological layer, this paper presents a high‐resolution lake‐level record for the period 4500–3500 cal. a BP. The collected data provide evidence of a complex palaeohydrological (climatic) oscillation spanning the ca. 4300–3850 cal. BP time interval, with major lake‐level maxima at ca. 4200 and 4050–3850 cal. a BP separated by a lowering episode around 4100 cal. a BP. The lake‐level highstands observed at Chatillon between 4300 and 3850 cal. BP appear to be synchronous with (i) a major flooding period recorded in deep cores from the large lakes Le Bourget and Bodensee, and (ii) glacier advance and tree line decline in the Alps. Such wetter and cooler climatic conditions in west‐central Europe around 4000 cal. a BP may have been a nonlinear response to decrease and seasonal changes in insolation. They may also provide a possible explanation for the general abandonment of prehistoric lake dwellings north of the Alps between 4360 and 3750 cal. a BP. Copyright © 2012 John Wiley & Sons, Ltd.