Abstract. Important intertidal sabellarian reefs are built by the polychaete Sabellaria alveolata (LINNÉ) in the Mont Saint‐Michel Bay in Normandy at Champeaux (France). Typical profiles and plans were done over a ten year period. At the same time, population dynamics were examined during the last six years. At this site a nearly complete morphological cycle spread itself over more than ten years. Initially built on rock substrate (primary settlement phase), the studied reefs actually lie on a sandy shore. The growth phase begins by stages of balls which are then joined with new settlements between or on older tubes (secondary settlement). This phase comes to an end with an important platform stage. Then, the destruction phase brings us back to dead eroded reefs on which new worms can settle (secondary settlement). This cycle seems to be independent of the age of the living population, although new settlements of young worms and also sufficient hydrodynamics seem necessary.

In order to understand the effect of the maize rhizosphere on denitrification, the diversity and the activity of the denitrifying community were studied in soil amended with maize mucilage. Diversity of the denitrifying community was investigated by polymerase chain reaction (PCR) amplification of total community DNA extracted from soils using gene fragments, encoding the nitrate reductase (narG) and the nitrous oxide reductase (nosZ), as molecular markers. To assess the underlying diversity, PCR products were cloned and 10 gene libraries were obtained for each targeted gene. Libraries containing 738 and 713 narG and nosZ clones, respectively, were screened by restriction fragment analysis, and grouped based on their RFLP (restriction fragment length polymorphism) patterns. In all, 117 and 171 different clone families have been identified for narG and nosZ and representatives of RFLP families containing at least two clones were sequenced. Rarefaction curves of both genes did not reach a clear saturation, indicating that analysis of an increasing number of clones would have revealed further diversity. Recovered NarG sequences were related to NarG from Actinomycetales and from Proteobacteria but most of them are not related to NarG from known bacteria. In contrast, most of the NosZ sequences were related to NosZ from alpha, beta, and gammaProteobacteria. Denitrifying activity was monitored by incubating the control and amended soils anaerobically in presence of acetylene. The N2O production rates revealed denitrifying activity to be greater in amended soil than in control soil. Altogether, our results revealed that mucilage addition to the soil results in a strong impact on the activity of the denitrifying community and minor changes on its diversity.

Different methods of microanalysis (energy of wavelength dispersive X-ray spectrometry microprobes, ion microanalyser) combined with histochemistry, allowed us to analyse the cement of the tube and the main glands connected to the building organ of Sabellaria alveolata (L.). These methods clearly showed a mineral component of which the main elements are phosphorus, probably linked to the organic component, calcium, and magnesium, with some manganese and iron. The biomineral is produced by glandular cells having "heterogeneous spherulae." The quick hardening of this cement probably takes place independent of and before the quinonic tanning of the inner organic lining of the wall of the tube.

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