Farming practices affect the soil microbial community, which in turn impacts crop growth and crop-weed interactions. This study assessed the modification of soil bacterial community structure by organic or conventional cropping systems, weed species identity [Amaranthus retroflexus L. (redroot pigweed) or Avena fatua L. (wild oat)], and living or sterilized inoculum. Soil from eight paired USDA-certified organic and conventional farms in north-central Montana was used as living or autoclave-sterilized inoculant into steam-pasteurized potting soil, planted with Am. retroflexus or Av. fatua and grown for two consecutive 8-week periods to condition soil nutrients and biota. Subsequently, the V3-V4 regions of the microbial 16S rRNA gene were sequenced by Illumina MiSeq. Treatments clustered significantly, with living or sterilized inoculum being the strongest delineating factor, followed by organic or conventional cropping system, then individual farm. Living inoculum-treated soil had greater species richness and was more diverse than sterile inoculum-treated soil (observed OTUs, Chao, inverse Simpson, Shannon, P < 0.001) and had more discriminant taxa delineating groups (linear discriminant analysis). Living inoculum soil contained more Chloroflexi and Acidobacteria, while the sterile inoculum soil had more Bacteroidetes, Firmicutes, Gemmatimonadetes, and Verrucomicrobia. Organically farmed inoculum-treated soil had greater species richness, more diversity (observed OTUs, Chao, Shannon, P < 0.05), and more discriminant taxa than conventionally farmed inoculum-treated soil. Cyanobacteria were higher in pots growing Am. retroflexus, regardless of inoculum type, for three of the four organic farms. Results highlight the potential of cropping systems and species identity to modify soil bacterial communities, subsequently modifying plant growth and crop-weed competition.

Plants alter soil biota which subsequently modifies plant growth, plant-plant interactions and plant community dynamics. While much research has been conducted on the magnitude and importance of soil biota effects (SBEs) in natural systems, little is known in agro-ecosystems. We investigated whether agricultural management systems could affect SBEs impacts on crop growth and crop-weed competition. Utilising soil collected from eight paired farms, we evaluated the extent to which SBEs differed between conventional and organic farming systems. Soils were conditioned by growing two common annual weeds: Amaranthus retroflexus (redroot pigweed) or Avena fatua (wild oat). Soil biota effects were measured in wheat (Triticum aestivum) growth and crop-weed competition, with SBEs calculated as the natural log of plant biomass in pots inoculated with living soil divided by the plant biomass in pots inoculated with sterilised soil. SBEs were generally more positive when soil inoculum was collected from organic farms compared with conventional farms, suggesting that cropping systems modify the relative abundance of mutualistic and pathogenic organisms responsible for the observed SBEs. Also, as feedbacks became more positive, cropweed competition decreased and facilitation increased. In annual cropping systems, SBEs can alter plant growth and crop-weed competition. By identifying the management practices that promote positive SBEs, producers can minimise the impacts of crop-weed competition and decrease their reliance on off-farm chemical and mechanical inputs to control weeds, enhancing agroecosystem sustainability.

Summary Understanding how weed communities assemble as a function of biotic and abiotic filters and transform through time has important implications for the sustainable management of agronomic systems. In a three‐year study, we evaluated weed community responses to lucerne (Medicago sativa, perennial) vs. continuous spring wheat (Triticum aestivum, annual, CSW) and weed management practices where weeds in the CSW system were managed with three contrasting approaches (herbicide, tillage or sheep grazing). Our results indicated no differences in weed diversity between the perennial and annual crops or across the different management practices in CSW. However, there were differences in weed community composition. Lucerne, with the exception of the establishing year, impeded the growth and reproduction of several annual weeds, including Amaranthus retroflexus, Thlaspi arvense, Lamium amplexicaule and Chenopodium album, but favoured perennial broad‐leaved weeds such as Taraxacum officinale and Cirsium arvense. The replacement of herbicide treatments in pre‐plant and post‐harvest in CSW with soil tillage or sheep grazing selected for different weed communities beyond the second year of establishment. The weed species driving the differences in CSW systems were Androsace occidentalis, more common in CSW managed chemically; Asperugo procumbens, more common in CSW managed with tillage; and T. officinale and Lactuca serriola, more common in CSW managed with sheep grazing. Understanding how cropping systems modify weed communities is a necessary step to shift from reactive weed control programmes to predictive management strategies.

Crop diversification and integration of livestock into cropping systems may improve the economic and environmental sustainability of agricultural systems. However, few studies have examined the integration of these practices in the semiarid areas of the Northern Great Plains (NGP). A 3-yr experiment was conducted near Bozeman, MT, to compare the effects of crop rotation diversity and weed management practices imposed during fallow periods [sheep (Ovis aries) grazing, reduced tillage, and conventional tillage] on spring wheat (Triticum aestivum L.) yields and weed pressure. Management treatments were applied to replicated whole plots, within which the split-plots received crop rotation treatments [continuous spring wheat (CSW) and a 3-yr rotation of annual forage, fallow, and spring wheat, where each phase was present in each year]. In the initial 2 yr, the realized rotational treatments were wheat-fallow and CSW. In the final year, wheat was grown following all phases of the diversified rotation. Yields were similar among management treatments within the wheat-fallow and CSW rotations. Weed pressure was generally low but perennial weeds were more abundant in grazing-managed, wheat-fallow systems. The integration of livestock into the annual hay crop-fallow-spring wheat rotation was associated with a nearly 30-fold increase in weed pressure and a yield reduction of 51.2% compared to conventional management. The results suggest that although targeted sheep grazing is a viable alternative to conventional fallow management in CSW and wheat-fallow rotations, successful integration of livestock in diversified cropping systems requires more effective weed management practices. ABStRACtCrop diversi cation and integration of livestock into cropping systems may improve the economic and environmental sustainability of agricultural systems. However, few studies have examined the integration of these practices in the semiarid areas of the Northern Great Plains (NGP). A 3-yr experiment was conducted near Bozeman, MT, to compare the e ects of crop rotation diversity and weed management practices imposed during fallow periods [sheep (Ovis aries) grazing, reduced tillage, and conventional tillage] on spring wheat (Triticum aestivum L.) yields and weed pressure. Management treatments were applied to replicated whole plots, within which the split-plots received crop rotation treatments [continuous spring wheat (CSW) and a 3-yr rotation of annual forage, fallow, and spring wheat, where each phase was present in each year]. In the initial 2 yr, the realized rotational treatments were wheat-fallow and CSW. In the nal year, wheat was grown following all phases of the diversi ed rotation. Yields were similar among management treatments within the wheat-fallow and CSW rotations. Weed pressure was generally low but perennial weeds were more abundant in grazing-managed, wheat-fallow systems. e integration of livestock into the annual hay crop-fallow-spring wheat rotation was associated with a nearly 30-fold increase in weed pres...

Although stand-alone and integrated management techniques have been cited as viable approaches to managing Canada thistle [Cirsium arvense(L.) Scop.], it continues to impact annual cropping and perennial systems worldwide. We conducted meta-analyses assessing effectiveness of management techniques and herbicide mechanism of action groups for controllingC. arvenseusing 55 studies conducted in annual cropping systems and 45 studies in perennial systems. Herbicide was the most studied technique in both types of systems and was effective at reducingC. arvense. However, integrated multitactic techniques, with or without herbicides, were more effective than sole reliance on herbicides for long-term control in both annual cropping and perennial systems. A variety of management techniques such as biocontrol, crop diversification, mowing, and soil disturbance provided control similar to that of herbicide. Our results suggest that many management techniques aimed at reducingC. arvensecan also improve crop yield or abundance of desired plants. This study highlights the need to devote more research to nonchemical and integrated management approaches forC. arvensecontrol.