Amoebiasis is the third leading cause of death from parasitic disease worldwide. The causative protozoan parasite, Entamoeba histolytica, is a potent pathogen. Propolis is a natural resinous substance collected by bees from vegetable sources and it has a therapeutic properties have been investigated in this work. So we evaluated the inhibitory activity of ethanolic extracts of propolis (EEP) on Entamoeba histolytica trophozoite growth. Propolis inhibited the growth of E.histolytica trophozoites and the level of inhibition varied according to the extract concentration and incubation times, it also showed a marked activity on cell lysis of trophozoite. The highest reduction of parasite growth was observed in culture exposed to (25,50 mg/ml) of propolis, in all incubation periods (24,48 )h. Growth reduction by 76 % was observed in 12.5 mg/ml propolis-treated culture, while the concentrations of (25, 50) mg/ml were able to inhibit growth by more than 90%. Light microscopic study showed morphological changes in E.histolytica trophozoites.

Entamoeba histolytica parasite was isolated from a stool sample, cultivated and maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM). The effect of two aqueous plant extracts (Hibiscus sabdariffa and Glycyrrhiza glabra) on the growth and activity of the parasite in the two culture media was investigated. The aqueous extracts of H. sabdariffa and G. glabra were effective in reducing the parasite size in the LEM medium. With respect to the reproduction rate, the third concentration (19.71 mg/ml) of H. sabdariffa was significantly effective in inhibiting such rate to 57.6 and 83.6% in LEM and LIAM media, respectively, while for G. glabra, no significant difference in the reproduction rate was observed in both culture media.

The study include isolation of 50 Pseudomonas aeruginosa from different clinical samples in Kirkuk city. The results indicate that the all isolates showed sensitivity to (Amikacin,Tobromycin, Imipenem, Pepracillin, and Aztreonam) 100% and92% to Ciprofloxacin and 90% to Carbenicillin. While the lowest sensitivity showed to be 12% against cefixim and Gentamicin. From these isolates only three showed to produce inducible beta lactamase .

he E. histolytica parasite was maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM). The effect of two calcium antagonists (Nifedipine and Ethylene-diaminetetraacetic acid EDTA) on the growth and activity of the parasite in the two culture media was investigated. The calcium antagonists Nifedipine and EDTA inhibited the reproduction rate of E. histolytica in a concentration-dependent manner. For Nifedipine, a concentration of 41.6 mg/ml inhibited the reproduction rate to 99.7% in both media. The EDTA had an approximate effect (98.2 and 95.8)% at a concentration of 0.83 mg/ml in LEM and LIAM media, respectively. Additionally, some cases of a parasite encystment were observed in LEM medium that was treated with Nifedipine.