Introduction: As recommended by World Health Organization, consumption of the fungal contaminated water does not cause to serious infection, but may lead to healthy or aesthetic problems. The aim of this research was to assess the occurrence of fungi in water and its relationship with fecal indicator bacteria. Materials and Methods: 110 water samples were collected from different location of water distribution systems in Aliabad-e Katul City, North of Iran during April to November 2018. Enumeration of coliforms bacteria and fungi were performed by multiple tube fermentation and membrane filtration method, respectively; as described in Standard Methods. Results: Our results showed that fungi and total coliform were detected in 31 (28.4%) and 26 (23.9%) samples, respectively. Among samples of fungi positive, 22 (20.2%), 7 (6.4%), and 5 (4.6%) water samples were positive for Aspergillus sp., Rhizopus sp., and Penicillium sp., respectively. However, none faecal coliform and E. coli were observed in all examined samples, proposing the absence of faecal pollution in water. The mean and SD residual chlorine and pH were 0.55 ± 0.23 (mg/l) and 7.30 ± 0.30, respectively. The statistical analysis showed a remarkable difference between the prevalence of total coliforms and fungal species (P < 0.001). Conclusion: Presence of potential opportunistic pathogens fungi in potable water can be considered as a health risk, especially for immuno-suppressed individuals. Therefore, cleaning the processes such as biofilm removal and addition of the free chlorine concentration can be effective to decrease fungi contamination and total coliform from water distribution system.

Background and objectives: Aspergillosis is a widely distributed infectious disease, which is difficult to manage. According to recent studies, the prevalence of resistant Aspergillus fumigatus has increased from 3.3% to 6.6%. Acquired triazole resistance in Aspergillus species is an evolving global health challenge, which has made the control of diseases caused by Aspergillus a concern. This study was performed to investigate prevalence of azole resistance in Aspergillus isolates from environmental samples. Methods: In this study, 316 soil samples were collected from three hospitals and a university campus in Gorgan (Iran) from July to September 2017. Two grams of each sample were suspended in 5 ml of 0.2M NaCl with 1% Tween 20. Then, 100 µl of the suspension was plated on sabouraud dextrose agar (SDA) supplemented with chloramphenicol, SDA supplemented with chloramphenicol and voriconazole (VOR, 1 mg/L) and SDA supplemented with chloramphenicol and itraconazole (ITC, 4 mg/L). The plates were incubated at 37 °C and examined for growth after 24, 48 and 72 hours. Results: We detected Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger and Aspergillus nidulans isolates in 187(59.2%), 84(26.6%), 147(46.5%) and 65(20.6%) samples, respectively. We found no VOR resistant isolate. However, 21 (25%) A. flavus and 16 (8.6%) A. fumigatus isolates were intermediate for VOR. In addition, seven (8.3%) A. flavus, 68 (36.4%) A. fumigatus, 41 (27.9%) A. niger and three (4.5%) A. nidulans isolates were resistant to ITC. Conclusion: We were able to detect A.fumigatus, A. flavus, A. niger from all four sampling sites in Gorgan, North of Iran. A. fumigatus is the most prevalent and most resistant isolate in the studied area. History of previous agriculture activity and use of pesticides in the proximity of sampling sites may have affected the rate of ITC resistance.