No abstract

Newly, the usage of nano bers (NFs) as wound dressings with the aim of their rapid healing and prevention of bacterial infection has been considered by researchers. In this regard, we produced the ethylcellulose/hydroxypropyl methylcellulose NFs incorporated with Aloe-vera (EC/HPMC/Alv) by the electrospinning technique. The produced NFs were investigated for their chemical structure, morphological, mechanical, thermal stability, degradation, swelling, cell viability, and antibacterial properties. Amongst the produced NFs, the NF samples containing 10% Alv illustrated the appropriate thermal stability and tensile properties. The produced NFs did not show any cell cytotoxicity which indicates their good compatibility. Also, NFs containing Alv signi cantly (P<0.05) increased cell proliferation and adhesion. In addition, the NFs/Alv sample was indicated antibacterial ability against S. aureus (10.21 ± 1.21 mm) and E. coli (5.06 ± 1.3 mm) pathogenic bacteria. As a result, these ndings suggest that the produced NFs could be applied as an active mat for wound dressing application.

Purpose: Mesoporous silica nanoparticles (MSNs) have drawn substantial interest as drug nanocarriers for breast cancer therapy. Nevertheless, because of the hydrophilic surfaces, the loading of well-known hydrophobic polyphenol anticancer agent curcumin (Curc) into MSNs is usually very low. Methods: For this purpose, Curc molecules were loaded into amine-functionalized MSNs (MSNs-NH2-Curc) and characterized using TGA, FTIR, FE-SEM, TEM, and BET. MTT assay and confocal microscopy, respectively, were used to determine the cytotoxicity and cellular uptake of the MSNs-NH2-Curc in the MCF-7 breast cancer cells. Besides, the expression levels of apoptotic genes were evaluated via qPCR and western blot. Results: It was revealed that MSNs-NH2 possessed high values of drug loading efficiency and exhibited slow and sustained drug release compared to bare MSNs. According to the MTT findings, while the MSNs-NH2-Curc were nontoxic to the human non-tumorigenic MCF-10A cells at low concentrations, it could considerably decrease the viability of MCF-7 breast cancer cells compared to the free Curc in all concentrations after 24, 48 and 72 h exposure times. A cellular uptake study using confocal fluorescence microscopy confirmed the higher cytotoxicity of MSNs-NH2-Curc in MCF-7 cells. Further, it was found that the MSNs-NH2-Curc could drastically affect the mRNA and protein levels of Bax, Bcl-2, caspase 3, caspase 9, and hTERT relative to the free Curc treatment. Conclusion: Taken together, these preliminary results suggest the amine-functionalized MSNs-based drug delivery platform as a promising alternative approach for Curc loading and safe breast cancer treatment.

Recently, the importance of biocompatible nanocomposite film with suitable properties has been further attracted for potential applications in the biomedical area. In this study, composite films of Gellan gum/Soy Protein/Cellulose...