Wheat is a staple food consumed by the majority of people in the world and its production needs to be doubled to feed the growing population. On the other hand, global wheat productivity is greatly affected due to drought and low fertility of soil under arid and semi-arid regions. Application of supplementary irrigation and plant growth-promoting rhizobacteria (PGPR) has been suggested as sustainable measures to combat drought stress and to improve soil fertility and, hence, crop yield. This research was undertaken to study the effect of supplementary irrigation together with a combination of various PGPR on the growth and yield of two wheat cultivars, namely Sardari and Sirvan. The results of variance analysis (mean of squares) showed that the effect of irrigation, cultivar, and irrigation and biofertilizer and irrigation on height, spike length, seed/spike, and numbers of spikes/m2, 1000-seed weight, and grain yield were significant at 1% probability level. The effect of cultivar and irrigation interactions showed that the highest grain yield was obtained in a treatment with two additional irrigations in Sirvan cultivar (5015.0 kg/ha) and Sardari (4838.9 kg/ha) as compared to the 3598 kg/ha and 3598.3 kg/h grain yield in Sirvan and Sardari cultivars with similar treatment, but without irrigation, i.e., dryland farming. Drought conditions significantly affected the wheat grain yield while supplementary irrigation resulted in 39.38% and 34.48% higher yields in Sirvan and Sardari cultivars.

To evaluate the effect of useful soil microorganisms and organic compounds on physiological characteristics and yield of two wheat cultivars under supplementary irrigation conditions, a study was conducted in the Agriculture Research Farm of Kurdistan University during the two cropping seasons of 2017–2018 and 2018–2019. A split–split plot-based study on a randomized complete block design with four replicates was used as an experimental design. The main factor was irrigation at three levels, including control without irrigation, supplementary irrigation in the booting stage, and supplementary irrigation in the booting and flowering stages. Two wheat cultivars, namely, Sardari and Sirvan, as sub-factors and application of bio-fertilizers in eight levels, including the use of bio-fertilizers containing: Mycorrhiza, Seaweed extract, Nitrozist and Phosphozist, Mycorrhiza + Nitrozist and Phosphozist, Seaweed extract + Nitrozist and Phosphozist, Mycorrhiza + Seaweed extract, Mycorrhiza + Nitrozist and Phosphozist + Seaweed extract, and non-application of bio-fertilizers, were considered as sub-factors. The results of both seasons of the experiment showed that the application of bio-fertilizers compared to the control treatment at all irrigation levels increased root volume, leaf relative water content (RWC), membrane stability index (MSI), and photosynthetic pigment content. The highest amount of H2O2, proline, and soluble carbohydrates were obtained in wheat under dry land conditions, and supplementary irrigation, especially two-time irrigation, significantly reduced the values of these traits. Supplementary irrigation also increased grain yield, so that in the conditions of two-time irrigation compared to the non-irrigation treatment (dry land), in the first and second seasons, the grain yield increased by 79.51 and 78.69%, respectively. Application of bio-fertilizers (Mycorrhiza + Nitrozist and Phosphozist + Seaweed extract) in comparison with the non-application of these fertilizers, due to increased root volume, RWC, MSI, and content of photosynthetic pigments, increased the grain yield in the first and second seasons of the experiment by 8.04 and 6.96%, respectively. As a result, suitable microorganisms and seaweed can improve wheat resistance mechanisms to water deficit, which along with using supplementary irrigation that saves water consumption improves plant growth and yield in areas faced with water shortage.

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IntroductionWheat (Triticum aestivum L.) is a strategic crop and one of the world's most essential cereals, providing most of the world's calories and protein needs. Drought stress is one of the main limitations for crop production such as wheat in arid and semi-arid regions. Plants can accumulate antioxidants, carbohydrates, and stress hormones that stimulate cell and molecular regeneration under stress conditions. Irrigation saves water, improves crop photosynthesis, and increases plant ability to absorb water and elements from soil. Therefore, irrigation at the right time or supplementary irrigation can help plant growth and crop yield under drought conditions. Appropriate nutrition with fertilizers increases plants' stress tolerance. Bio-fertilizers are restorative elements used in soil to improve tolerance to stresses such as drought stress. A well-known class of bio-fertilizers is plant growth promoting rhizobacteria (PGPR). These rhizosphere bacteria affect plant development and productivity by interacting with roots. Arbuscular mycorrhizal fungi (AMF) alleviate drought stress in plants by enhancing their ability to absorb water and nutrients from the soil. Seaweed extract bio-fertilizer is organic matter used to increase crop growth and soil fertility. This bio-fertilizer is utilized as growth stimulants and food supplements. Our research analyzed the effects of rhizobia and seaweed extracts on wheat's drought resistance mechanisms.Materials and methodsThis research was conducted in Iran in the crop years of 2017–2018 and 2018–2019 in the research farm of Kurdistan University Faculty of Agriculture located in Dehgolan with coordinates 47°18′ 55″ East and 35°19′ 10″ North with an altitude of 1866 meters above sea level, 45 kilometers east It was done on the wheat plant in Sanandaj city. The experiment was conducted in the form of a split-split plot in the form of a randomized complete block design with four replications. Irrigation treatments as the main factor (no irrigation or dry-land, one irrigation in the booting stage, two irrigations in the booting and spike stages), two wheat cultivars (Sardari and Sirvan) as secondary factors, and the application of biological fertilizers at eight levels including Mycorrhiza + Nitrozist and Phosphozist, Seaweed extract + Nitrozist and Phosphozist, Mycorrhiza + Seaweed extract, Mycorrhiza + Nitrozist and Phosphozist and no application of biological fertilizers (control) as Sub-sub-factors were considered.Results and discussionAccording to the study, when bio-fertilizer was applied with once and twice supplementary irrigation levels, leaf relative water content (RWC) and soluble protein content (SPC) increased, while lack of irrigation increased malondialdehyde (MDA). In both years, bio-fertilizers, especially their combinations, increased the amount and activity of enzymatic and non-enzymatic antioxidants, including peroxidase (POD), superoxide dismutase (SOD), phenol (Phe), flavonoid (Fla), and anthocyanin (Anth). Also, it enhanced the inhibition of free radicals by 2-2-Diphenyl picryl hydrazyl (DPPH) and cleared active oxygen species. It was found that malondialdehyde (MDA) levels were very low in wheat under two times irrigation with averages of 3.3909 and 3.3865 μmol g−1 FW. The results indicated a significant positive relationship between non-enzymatic and enzymatic antioxidants such as Phe, Fla, Anth, DPPH, POD, and SOD enzymes and their role in improving stress under dry-land conditions, especially in the Sardari variety. Biological fertilizers (Mycorrhiza + Nitrozist and Phosphozist + Seaweed extract) increased wheat yield compared to the control. Furthermore, Mycorrhiza + Nitrozist and Phosphozist + Seaweed extract improved grain yield by 8.04% and 6.96% in the 1st and 2nd years, respectively. Therefore, appropriate combinations of microorganisms, beneficial biological compounds, and supplementary irrigation can reduce the adverse effects of drought stress in arid and semi-arid regions.