Background: Ischemia reperfusion injury following acute ischemic insult is responsible for extension of injury.Bosentan is an endothelin receptor antagonist, which is currently used as a strong vasoconstrictor. This study aims to investigate the effects of bosentan on ischemia reperfusion injury after brain ischemic stroke in rat model. Methods: Forty male Wistar rats were randomly allocated into four study groups: Sham group: Rats underwent the anesthesia & surgery for an identical period to the other 3 study groups without intervention. Control group: Rats underwent anesthesia & surgery including bilateral common carotid artery ligation (BCCAL) for 30 minutes and then reperfusion for 1 hour. Vehicle group: Four days before ischemia, rats were administered with a vehicle (5% gummi arabicum) and then anesthesia &BCCAL surgery were performed. Bosentan treat group: Four days before Al-Aubaidy et al (2019): Bosentan effect in brain ischemia September 2019 Vol. 22(IV) ©Annals of Tropical Medicine & Public Health SPe145ischemia, rats were administered with bosentan (100mg/ kg/day) and then anesthesia & BCCAL surgery were performed.Results: Bosentan treated group exhibited lower concentration of interleukin 6 (IL-6)in the brain (2168.0±30.67 pg/mL) than in the control group (2571.37±96.58 pg/mL) (P≤0.05). In addition, interleukin 10 (IL-10) levels were significantly high in the bosentan group (275.7±15.97 pg/mL) when compared to the control group (244.05±12.23 pg/mL) (P≤0.05). This was associated with a non-significant reduction in the brain levels of endothelia nitric oxide synthase (eNOS) in the bosentan treated groups (90.23±1.14 ng/mL) when compared to the control group (90.94±2.48), (P>0.05). Conclusion:Bosentan treatment have protective effects against the inflammatory damage following ischemia reperfusion injury following acute myocardial infarction.