Antibiotic resistance bacteria has become a worldwide problem. The shortage of new antibiotics has prompted research into chemicals that could act as adjuvants and enhance the efficacy of available antibiotics. Bacterial susceptibility to antibiotics was determined using the e-test method to evaluate the effect of commonly used antibiotics on the selected isolate (S20). The isolate displayed resistance to cefoxitin, benzylpenicillin, oxacillin, erythromycin, ciprofloxacin, and azithromycin. Whereas it was susceptible to the rest antibiotics. In this study, we reported the draft genome sequence of S20, isolated from a patient. The genome consists of 4,557,070 bp, with a GC of 46.4%. It has 228 RNA reads that protein-coding genes encoding multidrug resistance transporters, virulence factors. The draft genome sequences project was deposited in GenBank under accession no.. PRJNA480592. The version described in this paper is the first version. To estimate the phylotypes in the selected genomes, the 16S rRNA gene sequences were retrieved from the RAST annotation and used as a query against the SILVA reference database with the threshold set to above 97%. Bacillus mycoides was the closest genus to our isolate (100%).