The interaction between surface water represented by Tigers River, Fish Lake, drainage and groundwater was examined in this study. Samples were collected from Al-Azezeyia city for stable isotope (2H and18O). Samplings of water were taken during January – September 2016 during the period of breeding fish. Two samples were taken from a fish lake, three samples from Tigers River and one sample from water drainage. The aim of this research is to assess the interaction of a fish lake on surface water by using isotopic techniques. The values of stable isotopes of 18O range-7.78 – 8.10‰, -7.43 – -8.11‰, -3.2 – 3.3‰ and -6.0 – 6.2‰. The values of Deuterium range -45 – 45.56‰, -46.7 – -45.72‰, -37.87 – 37.98‰ and -28.4 – -29.7‰ in the fish lake, Tigris river, groundwater and water drainage, respectively. The results show there is no effect of interaction between the fish lake and Tigers River due to a short distance between the river and the lakes, lack evaporation effects and high flow rate.