Background: Sepsis is a life-threatening condition that arises when the body's response to infection causes injury to its own tissues and organs. Materials and methods: This was cross sectional study conducted at Baraah pediatric center, Khartoum, Sudan during the period from May 2022 to August 2022, to measure protein C level in Sudanese children and infants with Sepsis. 50 patients were selected as a case group (46% were female and 54% were male.), and apparently healthy donors were selected as control group. 2.8 ml of venous blood samples were collected in Tri Sodium Citrate Anticoagulant. CRP and the blood culture were done for all the patients. The protein C level was performed using Ichroma device. Results: Protein C results revealed the mean of protein C in case was (63.7±8.9 ), and in the control group  was(79.8±11.1 ),  when compared protein C mean  between case and control groups there was a  highly significant decreased with ( p value 0.00) Also in the case group there was an insignificant differences between protein C,  age, gender, and culture,  p value >0.05),  . In addition the result showed insignificant correlation between protein C and CRP(p value ≥0.05). The most common types of isolated bacteria in sepsis patients was Klebsiella Conclusion: The study concludes that there is a significant decrease in protein C level in patients with sepsis. Keywords: protein C , CRP sepsis , bacteria , Klebsiella