The present study is aimed to evaluate the efficacy of Nerium indicum and Leucas aspera leaf extract and fractions for anti-arthritic activity using invitro inhibition of protein denaturation model and anti-inflammatory activity using albumin denaturation assay comparing with Diclofenac sodium used as a standard drug. Antiarthritic activity was carried out using 10-80 µg/ml concentration, whereas antiinflammatory activity at the concentration of 25-100 µg/ml. The results of the present study represents that the methanolic extract and fractions of both the plants demonstrates dose dependent inhibition. The maximum antiarthritic activity of Nerium indicum and Leucas aspera was found at the concentration of 80 µg/ml with 81.6% and 86.2% inhibition. Similarly, maximum antiinflammatory activity of Nerium indicum and Leucas aspera was observed at the concentration of 100 µg/ml with 84.4 and 87.2% inhibition. It is evident that the cause of inflammation and arthritis is mainly due to denaturation of tissue proteins and the present study expresses that denaturation of proteins is inhibited, inhibiting the progression of arthritis and reducing the cause of inflammation.